Inhibition of IGF1R activity enhances response to trastuzumab in HER-2-positive breast cancer cells

Annals of Oncology : Official Journal of the European Society for Medical Oncology
Brigid C BrowneNorma O'Donovan

Abstract

although trastuzumab has improved the prognosis for HER-2-positive breast cancer patients, not all HER-2-positive breast tumours respond to trastuzumab treatment and those that initially respond frequently develop resistance. Insulin-like growth factor-1 receptor (IGF1R) signalling has been previously implicated in trastuzumab resistance. We tested IGF1R inhibition to determine if dual targeting of HER-2 and IGF1R improves response in cell line models of acquired trastuzumab resistance. HER-2, IGF1R, phospho-HER-2, and phospho-IGF1R levels were measured by enzyme-linked immunosorbent assays in parental and trastuzumab-resistant SKBR3 and BT474 cells. IGF1R signalling was targeted in these cells using both small interfering RNA (siRNA) and the tyrosine kinase inhibitor, NVP-AEW541. IGF1R levels were significantly increased in the trastuzumab-resistant model, SKBR3/Tr, compared with the parental SKBR3 cell line. In both the SKBR3/Tr and BT474/Tr cell lines, inhibition of IGF1R expression with siRNA or inhibition of tyrosine kinase activity by NVP-AEW541 significantly increased response to trastuzumab. The dual targeting approach also improved response in the parental SKBR3 cells but not in the BT474 parental cells. our results co...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P CarterH M Shepard
Dec 26, 2001·Journal of the National Cancer Institute·Y LuM Pollak
Mar 31, 2004·Cancer Cell·Carlos García-EcheverríaFrancesco Hofmann
May 29, 2007·Oncogene·R Nahta, F J Esteva
Aug 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christoph A RitterCarlos L Arteaga
Jul 22, 2008·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A Esparís-OgandoA Pandiella
May 16, 2009·Current Cancer Drug Targets·B C BrowneN O'Donovan
May 27, 2010·Molecular Cancer Therapeutics·Neil A O'BrienDennis J Slamon

❮ Previous
Next ❯

Citations

Mar 15, 2012·Investigational New Drugs·Andrea GombosAhmad Awada-Hussein
Oct 12, 2012·Journal of Mammary Gland Biology and Neoplasia·Yuzhe Yang, Douglas Yee
Mar 31, 2012·Science Translational Medicine·Howard M Stern
Jul 26, 2012·Chemotherapy Research and Practice·Rita Nahta
Apr 28, 2011·Molecular Cancer Research : MCR·Kathryn TworkoskiDavid F Stern
Sep 14, 2012·Therapeutic Advances in Gastroenterology·Alicia F C Okines, David Cunningham
Oct 24, 2013·World Journal of Gastroenterology : WJG·Michael LuisRamon Andrade de Mello
May 28, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Alessandra FabiOreste Segatto
Sep 26, 2013·Nature Reviews. Clinical Oncology·Roopma WadhwaJaffer A Ajani
Nov 3, 2012·Breast Cancer Research and Treatment·Brigid C BrowneNorma O'Donovan
May 4, 2012·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Sean C HarringtonPaul Haluska
Jun 25, 2014·Molecular Cancer·Martina Sj McDermottNorma O'Donovan
Apr 2, 2016·Cancer Cell International·Elena PeraGregory R Monteith
Mar 31, 2012·Journal of Immunotoxicology·Zhou LuqiuCai Yingqian
Mar 12, 2011·MAbs·Jonathan Fitzgerald, Alexey Lugovskoy
Jun 19, 2012·Cancer Treatment Reviews·Alberto OcañaAtanasio Pandiella
Feb 13, 2015·Frontiers in Oncology·Xiaochun WangJia-Lin Yang
Sep 7, 2016·Frontiers in Cell and Developmental Biology·Sean P KennedyDavid R Croucher
Sep 19, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Morteza MotallebnezhadMehdi Yousefi
Oct 14, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ling MaoJin-Hai Tang
Dec 17, 2016·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Rashmi R Shah
Sep 10, 2014·Cancers·Genevieve HousmanSibaji Sarkar
Nov 4, 2017·International Journal of Molecular Sciences·Andrea NicoliniAngelo Carpi
Dec 18, 2012·Current Opinion in Obstetrics & Gynecology·Gottfried E Konecny
Mar 25, 2018·Blood·Malik Bisserier, Narendra Wajapeyee
Jan 7, 2020·Current Drug Metabolism·Kanisha Shah, Rakesh M Rawal
Jan 30, 2016·Oncotarget·Debora de Melo GagliatoGabriel N Hortobagyi
Sep 24, 2011·Expert Review of Hematology·Annalisa Chiappella, Umberto Vitolo
Apr 30, 2017·Nature Communications·Esther LatresJesper Gromada
Jan 5, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael Pollak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved