Inhibition of IL-2 receptor induction and IL-2 production in the human leukemic cell line Jurkat by a novel peptide inhibitor of protein kinase C

Cellular Immunology
C G IoannidesC A O'Brian

Abstract

We recently reported that the myristoylated peptide N-myristoyl-Lys-Arg-Thr-Leu-Arg (N-m-KRTLR) is a novel protein kinase C inhibitor. In this study, we investigated the biological effects of N-m-KRTLR using as an in vitro model the induction of the IL-2 receptor and IL-2 secretion by Jurkat cells in response to stimulation with 12-O tetradecanoylphorbol-13-acetate (TPA) plus phytohemagglutinin (PHA) and TPA plus OKT3 mAb. N-m-KRTLR significantly suppressed induction of the IL-2 receptor on the surface of the Jurkat cells by TPA plus either PHA or OKT3 mAb. Furthermore, N-m-KRTLR inhibited the production and release of IL-2 from cultured Jurkat cells stimulated with TPA plus either PHA or OKT3 mAb. Similarly, this peptide significantly inhibited the IL-2 production in normal human peripheral blood mononuclear cells in response to stimulation by TPA and PHA. In contrast, this peptide did not affect expression of the CD3 complex on the surface of the Jurkat cells either alone or in the presence of TPA or PHA. Furthermore, N-m-KRTLR did not interfere with the spontaneous proliferation of the Jurkat cells, and its effects on IL-2 secretion and IL-2 receptor expression in the Jurkat cells were evident without loss of cell viability....Continue Reading

References

Jan 1, 1990·Biochemical Pharmacology·C A O'BrianJ H van Boom
Oct 1, 1989·Cellular Immunology·C G IoannidesC D Platsoucas
Aug 1, 1989·Molecular and Cellular Endocrinology·P J ParkerC Webster
Dec 1, 1989·Cancer Metastasis Reviews·C A O'Brian, N E Ward
Sep 9, 1985·Biochimica Et Biophysica Acta·C L Ashendel
Dec 21, 1988·Journal of the National Cancer Institute·C A O'BrianB W Anderson
Jun 22, 1984·Science·D A Cantrell, K A Smith

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Citations

Mar 4, 1999·European Journal of Immunology·M A López-LagoP Barja
Nov 30, 2007·Biochemistry·Allison R NelsonChristopher E Sims
Sep 27, 2005·Biochimica Et Biophysica Acta·Marie A BogoyevitchAlbert J Ketterman
Jun 20, 2008·Organic & Biomolecular Chemistry·Kelly M StewartShana O Kelley
Aug 20, 2020·Biomaterials Science·Jiranuwat SapudomJeremy C M Teo
Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·C G IoannidesR S Freedman

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