PMID: 7539723Jun 1, 1995Paper

Inhibition of IL-4 receptor up-regulation on B cells by antisense oligodeoxynucleotide suppresses IL-4-induced human IgE production

Clinical and Experimental Immunology
K IkizawaY Yanagihara

Abstract

IL-4 is shown to up-regulate its own receptor (IL-4R) on human lymphocytes, but the functional significance of up-regulated IL-4R is not clear regarding IgE production. This study investigated the possible role of IL-4-induced up-regulation of IL-4R on B cells in the induction of human IgE synthesis by means of antisense strategy. Among three antisense oligodeoxynucleotides designed against the downstream of translation initiation site of IL-4R cDNA, S-oligo 1, complementary to nucleotide 1-24, showed the strongest inhibition of the constitutive expression of IL-4R on Daudi cells. Addition of S-oligo 1 together with IL-4 also decreased the up-regulated but not constitutive levels of IL-4R on peripheral blood B cells without affecting the concomitant enhancement of CD23, CD40, HLA-DR and surface IgM expression, indicating that its effect is specific for IL-4R up-regulation. When S-oligo 1 was added to B cells costimulated with IL-4 and anti-CD40 MoAb, it induced a dose-dependent inhibition of IgE production. This inhibition was accompanied by a decrease in the expression of mature C epsilon transcripts, whereas the accumulation of germ-line C epsilon transcripts was not affected by S-oligo 1. These data suggest that the signal t...Continue Reading

References

May 7, 1992·Nature·R J ArmitageC R Maliszewski
Jan 1, 1992·International Archives of Allergy and Immunology·Y YanagiharaT Shida
Jan 30, 1992·The New England Journal of Medicine·D S RobinsonA B Kay
Nov 1, 1991·Immunology Today·M L KapsenbergH M Jansen
Mar 1, 1991·The Journal of Clinical Investigation·C Y WuG Delespesse
Jan 1, 1990·International Immunology·R J ArmitageW C Fanslow
Jan 1, 1990·International Immunology·J P GalizziA Miyajima
Mar 1, 1990·European Journal of Immunology·C E ZuberJ Banchereau
Sep 1, 1990·Cellular Immunology·C E ZuberJ Banchereau
Mar 1, 1987·The Journal of Allergy and Clinical Immunology·Y YanagiharaG Delespesse
Aug 1, 1989·European Journal of Immunology·A ValléJ Banchereau
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G ThyphronitisF D Finkelman
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K A Smith, D A Cantrell
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J M DepperW C Greene
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L XuP Rothman

❮ Previous
Next ❯

Citations

Oct 5, 2011·European Journal of Immunology·G L MoschovakisReinhold Förster
Jun 1, 1995·Clinical and Experimental Immunology·R Q Zheng, D M Kemeny
Aug 11, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Akinori KanaiSumio Sugano
Mar 3, 1998·Critical Reviews in Oncology/hematology·L M TuytE Vellenga
Jan 24, 1998·The Journal of Allergy and Clinical Immunology·Y YanagiharaK Ikizawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.