PMID: 2499652Jun 1, 1989Paper

Inhibition of immunoglobulin gene rearrangement by the expression of a lambda 2 transgene

The Journal of Experimental Medicine
J HagmanU Storb

Abstract

The rearrangement of Ig genes is known to be regulated by the production of H and kappa L chains. To determine whether lambda L chains have a similar effect, transgenic mice were produced with a lambda 2 gene. It was necessary to include the H chain enhancer, since a lambda gene without the added enhancer did not result in transgene expression. The lambda 2 transgene with the H enhancer was expressed in lymphoid cells only. The majority of the B cells of newborn transgenic mice produced lambda, whereas kappa + cells were reduced. Concomitantly, serum levels of kappa and kappa mRNA were diminished. By 2 wk after birth the proportion of kappa-expressing cells was dramatically increased. Adults had reduced proportions of B cells that produced lambda only, but the levels of lambda were still higher than in normal littermates. Also, kappa + cells were still lower than in normal mice. Analysis of hybridomas revealed that reduction of kappa gene rearrangement was the basis for the decreased frequency of kappa + cells. Furthermore, many cells also contained an unrearranged H chain allele. It was concluded that feedback inhibition by the lambda 2 together with endogenous H protein may have inhibited recombinase activity in early pre-B c...Continue Reading

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Citations

Feb 28, 1998·Immunologic Research·J EriksonJ H Roark
Dec 23, 2004·Springer Seminars in Immunopathology·V Michael Holers
Aug 1, 1990·The Journal of Experimental Medicine·T J Vasicek, P Leder
May 1, 1992·The Journal of Experimental Medicine·T J VasicekP Leder
Mar 1, 1995·The Journal of Experimental Medicine·P E RothU Storb
Jun 1, 1990·Immunological Reviews·U Storb
Feb 1, 1994·Leukemia & Lymphoma·N B Stiernholm, N L Berinstein
Aug 24, 2010·Immunological Reviews·Christian Vettermann, Mark S Schlissel
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin J MarchbankV Michael Holers

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