Inhibition of immunosuppressive effects of melanoma-inhibiting activity (MIA) by antisense techniques

International Journal of Cancer. Journal International Du Cancer
Piotr JachimczakU Bogdahn

Abstract

Melanoma inhibitory activity (MIA) is an 11 kD protein secreted by malignant melanomas. Recent studies revealed an interaction of MIA with epitopes of extracellular matrix proteins including fibronectin. Structural homology of MIA with the binding sites of alpha4beta1 integrin results in complex interactions of MIA with molecules binding to alpha4beta1 integrin. As cells of the immune system express alpha4beta1 integrins (VLA-4), we investigated whether MIA may modulate the function of human leukocytes. Here we describe the effects of MIA on the activation of human PBMCs and auto-/allogeneic lymphokine-activated killer cell (LAK) cytotoxicity in human MIA-negative glioma cell lines and MIA-positive melanoma cell lines in vitro. MIA inhibits PHA- or IL-2-induced human PBMC proliferation in a dose-dependent manner up to 63% ((3)H-Tdr incorporation) and 59% (cell count), respectively, when added to the cell culture prior to mitogen stimulation. In addition, both autologous (GL and HW) and allogeneic (HTZ-17, HTZ-243 and HTZ-374) antitumor LAK cytotoxicity was reduced by the addition of exogenous rhMIA (500 ng/ml, f.c.). Consequently, endogenous inhibition of MIA expression in human melanoma cells by MIA-specific phosphorothioate a...Continue Reading

References

Mar 1, 1992·Immunology Today·Y ShimizuS Shaw
Aug 3, 1994·Journal of the National Cancer Institute·S A RosenbergD E White
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·J C LougheedT M Handel
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D M RoseM H Ginsberg
Sep 29, 2001·Immunology·C Smith, V Cerundolo
Jul 20, 2002·The Journal of Urology·A G Kennedy-SmithD N J Hart
Nov 15, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Anja-Katrin BosserhoffTad A Holak

❮ Previous
Next ❯

Citations

Mar 26, 2014·International Journal of Clinical Oncology·Tomonori SasahiraHiroki Kuniyasu
Jun 24, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Tomonori SasahiraHiroki Kuniyasu
Jan 1, 2014·Experimental Dermatology·Alexander Riechers, Anja Katrin Bosserhoff
Apr 15, 2018·Pathology International·Tomonori SasahiraTadaaki Kirita
Mar 21, 2013·British Journal of Cancer·M KuriharaH Kuniyasu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Related Papers

The Journal of Biological Chemistry
Richard BauerAnja-Katrin Bosserhoff
Biological Chemistry
R StollAnja-Katrin Bosserhoff
International Journal of Cancer. Journal International Du Cancer
Jennifer Schmidt, Anja-Katrin Bosserhoff
© 2021 Meta ULC. All rights reserved