PMID: 6972363May 1, 1981Paper

Inhibition of induced ornithine decarboxylase activity in Chinese hamster cells by gamma radiation, far ultraviolet light and psoralen plus near-ultraviolet light: a comparative study

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
E Ben-Hur, E Riklis

Abstract

Inhibition by radiation of the transcriptionally controlled induction of ornithine decarboxylase (ODC) was studied in plateau-phase Chinese hamster cells. The effects of gamma radiation, far ultraviolet (254nm, UV-C) light and psoralen plus near-U.V. light (360nm, PUV-A) were compared. Increasing doses of all three treatments caused an exponential decrease in the capacity for ODC induction. This was preceded by a large shoulder region in the case of PUV-A. There was no shoulder for gamma radiation and possibly none or a small one for UV-C. When compared on the basis of cell killing UV-C is 13 times more efficient in inhibiting ODC induction than PUV-A and 69 times more than gamma radiation. The doses required to produce an inactivating hit for inhibition of ODC induction (D0) are 106 krad for gamma radiation, 2400 J m-2 for PUV-A and 14 J m-2 for UV-C. These doses produce about one gamma ray lesion, one psoralen adduct and one pyrimidine dimer in the structural gene of ODC. However, the number of lesions per transcription unit may be up to 10, depending on the contribution to the effect by lesions in transcribed and non-transcribed spacers and introns. Thus, assuming that most of the inhibition is due to effects on RNA synthesi...Continue Reading

References

Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·U Bachrach
Jan 1, 1978·Annual Review of Genetics·W Sauerbier, K Hercules
Jan 25, 1975·Journal of Molecular Biology·P B Hackett, W Sauerbier
Jan 1, 1972·Annual Review of Biophysics and Bioengineering·R B Setlow, J K Setlow
Apr 14, 1969·Journal of Molecular Biology·H Michalke, H Bremer
Apr 1, 1971·Photochemistry and Photobiology·E Ben-Hur, R Ben-Ishai
May 30, 1967·Biochimica Et Biophysica Acta·A Ishihama, T Kameyama
Jan 1, 1970·Biochimica Et Biophysica Acta·W SauerbierP B Hackett
Mar 25, 1971·Biochimica Et Biophysica Acta·R C van den BosR J Planta
May 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·E Ben-HurE Riklis
Dec 13, 1978·Journal of Clinical Engineering·C E McCullough, L S Baker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.