Inhibition of inducible nitric oxide synthase expression by interleukin-4 and interleukin-13 in human lung epithelial cells

Immunology
N BerkmanK F Chung

Abstract

Nitric oxide produced by the inducible enzyme, nitric oxide synthase (iNOS), is implicated in immunological and inflammatory processes. We determined the effects of T-helper (Th)2-derived cytokines on the induction of iNOS from an epithelial A549 cell line and human airway epithelial cells stimulated by a mixture of interleukin-1 beta (IL-1 beta), interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha). Interleukin-4 (IL-4) and interleukin-13 (IL-13) but not interleukin-10 (IL-10) inhibited both iNOS mRNA expression and nitrite release in A549 cells. On human airway epithelial cells, IL-4 and IL-13 reduced iNOS mRNA expression. Dexamethasone also inhibited both iNOS expression and nitrite release. Th2 cytokines, IL-4 and IL-13, inhibit iNOS upregulation by Th1 cytokines, indicating an important reciprocal role of Th1 and Th2 T-cell subsets on lung epithelial cells.

References

Feb 14, 1992·Biochemical and Biophysical Research Communications·F Q CunhaF Y Liew
Jan 1, 1992·Annual Review of Immunology·A Sher, R L Coffman
May 1, 1991·The Journal of Clinical Investigation·Q HamidP K Jeffery
Dec 1, 1989·The Journal of Experimental Medicine·D F FiorentinoT R Mosmann
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J Stuehr, M A Marletta
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs
Feb 15, 1994·Biochemical and Biophysical Research Communications·R A RobbinsJ Polak
Aug 30, 1994·Biochemical and Biophysical Research Communications·R A RobbinsJ M Polak
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·K AsanoJ S Stamler
Mar 1, 1995·Immunology Today·P J Barnes, F Y Liew
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
Aug 1, 1993·American Journal of Respiratory Cell and Molecular Biology·T J StandifordR M Strieter

❮ Previous
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Citations

Nov 9, 2001·Biochemical and Biophysical Research Communications·B M BrooksJ W Coleman
Feb 12, 2000·The Journal of Allergy and Clinical Immunology·S T HolgateJ L Lordan
Dec 1, 1999·Psychiatry Research· van Kammen DPJ K Yao
Sep 28, 2000·International Journal of Pediatric Otorhinolaryngology·W LiS K Juhn
Oct 30, 1998·Molecular Medicine Today·P J Barnes, S Lim
Jul 9, 2002·Clinical and Experimental Immunology·J W Coleman
Aug 8, 2002·Parasite Immunology·Jolanta M DzikElzbieta Wałajtys-Rode
Oct 29, 1998·Mediators of Inflammation·V H van der Velden
Aug 17, 2002·The Pediatric Infectious Disease Journal·Frank DeStefanoUNKNOWN Vaccine Safety Datalink Research Group
Aug 24, 1999·Thorax·K F Chung, P J Barnes
Mar 11, 2000·American Journal of Respiratory and Critical Care Medicine·P J Barnes
Feb 17, 2001·American Journal of Respiratory and Critical Care Medicine·Y J KaoG N Colasurdo
Jun 21, 2001·American Journal of Respiratory Cell and Molecular Biology·A C WangD J Conrad
Jan 24, 2002·American Journal of Respiratory Cell and Molecular Biology·Rajeev VenkayyaDavid J Erle
Mar 10, 2007·American Journal of Respiratory Cell and Molecular Biology·Vinod SureshSteven C George
Jan 21, 2009·Respiratory Research·Jingjing JiangSteven C George
Jan 23, 1999·International Reviews of Immunology·P Chomarat, J Banchereau
Aug 29, 2007·Biochemical and Biophysical Research Communications·Lifang ShaoDavid A Geller
Apr 4, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K ChibanaH W Chu
Oct 7, 2004·Trends in Molecular Medicine·Talal A Chatila
Jul 25, 2012·Allergology International : Official Journal of the Japanese Society of Allergology·Mitsuru Munakata
Feb 7, 2002·Nitric Oxide : Biology and Chemistry·Philip E JamesHarold M Swartz
Apr 28, 2016·Clinical and Experimental Pharmacology & Physiology·D GiglioL Ny
Jan 31, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas J GrossMartha M Monick
Aug 24, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·T KubotaJ M Krueger
Jul 30, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amarjit S NauraA Hamid Boulares
May 17, 2014·International Surgery·Susumu SaigusaMasato Kusunoki

❮ Previous
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