Inhibition of inducible nitric oxide synthase gene expression by glucocorticoid-induced protein(s) in lipopolysaccharide-stimulated J774 cells

European Journal of Pharmacology
F D'AcquistoR Carnuccio

Abstract

Glucocorticoids inhibit inducible-type NO synthase activity in a variety of cell types. We report here that proteins recovered from the medium of dexamethasone-treated J774 macrophages (1, 10, 100 microg/ml) inhibited lipopolysaccharide-stimulated nitrite generation by 10.0 +/- 3.0%, 32.3 +/- 5.3% and 55.0 +/- 6.0%, respectively, and inducible NO synthase mRNA expression in these cells. Immunoblotting analysis of crude and partially purified glucocorticoid-induced proteins with an anti-lipocortin-1 polyclonal antiserum revealed the presence of lipocortin-1-like immunoreactive species with a molecular mass of 35-37 kDa. Furthermore, inhibition of lipopolysaccharide-induced nitrite production by glucocorticoid-induced proteins in J774 cells was reversed by addition of anti-lipocortin-1 neutralizing polyclonal antibody (1:60 dilution; 4 h before lipopolysaccharide). Comparison of glucocorticoid-induced proteins inhibition of both nitrite production and inducible NO synthase mRNA expression suggests that these effects result mainly from inhibition of lipopolysaccharide-mediated inducible NO synthase gene expression. These results indicate that negative regulation of inducible NO synthase by glucocorticoids is, at least in part, med...Continue Reading

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Citations

Mar 10, 2009·Annals of Biomedical Engineering·Mark ChenDean Ho
Nov 5, 2011·BMC Complementary and Alternative Medicine·Joseph SchwagerNathalie Richard
Nov 19, 2003·The European Journal of Neuroscience·Sabine GoldeAlastair Compston
Feb 11, 2015·British Journal of Pharmacology·P G TrentinP M R Silva
Apr 14, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joseph SchwagerDaniel Raederstorff
Jun 18, 2005·Memórias do Instituto Oswaldo Cruz·Ahmad M KamalMauro Perretti
Sep 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·Liqiang Ren, Peter J Syapin

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