Inhibition of inflammatory nitric oxide production and epidermis damages by Saccharomycopsis Ferment Filtrate

Journal of Dermatological Science
Hsiou-Hsin TsaiShing-Chuan Shen

Abstract

Yeast extracts have been shown to perform anti-inflammatory and cytoprotective activities. However, the effects of yeast extracts on lipopolysaccharide (LPS)-induced nitric oxide (NO) production and epidermal damages are still unclear. To investigate the effect of Saccharomycopsis Ferment Filtrate (SFF) on LPS-induced NO production in RAW264.7 macrophages and epidermal damages. RAW264.7 cells are incubated with LPS (25 ng/mL) and different concentrations of SFF. The amount of NO production is detected by Griess reaction. Additionally, the expression of inducible nitric oxide synthase (iNOS) and heme oxygenase-1 (HO-1) are detected by Western blotting. Artificial epidermis is also used to mimic the in vivo condition to investigate the protective effects of SFF on LPS- or ultraviolet radiation (UVR)-induced damages by histology and electron microscopy. The results show that SFF addition inhibits LPS-induced NO production and iNOS protein expression in a concentration-dependent manner without notable cytotoxicity in RAW264.7 cells, and induction of HO-1 protein expression by SFF was observed. Interestingly, both HO-1 inducers, hemin and CoCl2, significantly attenuated LPS-induced NO production and iNOS protein expression. The addi...Continue Reading

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Citations

Sep 27, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chin-Feng ChanYung-Sheng Lin
Dec 4, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tilak KhanalHye Gwang Jeong

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