PMID: 8947488Nov 15, 1996Paper

Inhibition of insulin release by synthetic peptides shows that the H3 region at the C-terminal domain of syntaxin-1 is crucial for Ca(2+)- but not for guanosine 5'-[gamma-thio]triphosphate-induced secretion

The Biochemical Journal
F MartinJ A Reig

Abstract

Recently, we have described the presence and possible role of syntaxin in pancreatic beta-cells by using monoclonal antibodies [F. Martin, F. Moya, L. M. Gutierrez, J.A. Reig, B. Soria (1995) Diabetologia 38, 860-863]. In order to characterize further the importance of specific domains of this protein, the functional role of a particular region of the syntaxin-1 molecule has now been investigated by using two synthetic peptides, SynA and SynB, corresponding to two portions of the H3 region at the C-terminal domain of the protein, residues 229-251 and 197-219 respectively. Functional experiments carried out in permeabilized pancreatic beta-cells demonstrate that these peptides inhibit Ca(2+)-dependent insulin release in a dose-dependent manner. This effect is specific because peptides of the same composition but random sequence do not show the same effect. In contrast with this inhibitory effect on Ca(2+)-induced secretion, both peptides increase basal release. However, under the same conditions, SynA and SynB do not affect guanosine 5'-[gamma-thio]triphosphate-induced insulin release. These results demonstrate that specific portions of the H3 region of syntaxin-1 are involved in critical protein-protein interactions specificall...Continue Reading

Citations

Mar 20, 2002·Nature Cell Biology·Anke DiDeborah J Nelson
Aug 16, 2005·Molecular Endocrinology·Beth A Spurlin, Debbie C Thurmond
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·S BargF Thévenod
Sep 23, 2020·Journal of Cellular Physiology·Bor L Tang
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·S N YangP O Berggren
Feb 29, 2008·Cells, Tissues, Organs·B SoriaF Martin
Feb 1, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Satoru NogamiHiromichi Shirataki
Dec 17, 2003·Journal of Neurochemistry·Clara Blanes-MiraAntonio Ferrer-Montiel
Oct 5, 2010·HFSP Journal·Bernat SoriaIvan Quesada
Sep 1, 2000·Seminars in Cell & Developmental Biology·R A Easom
Jul 29, 1998·General and Comparative Endocrinology·J L QuintanarJ A Reig

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