PMID: 11926897Apr 3, 2002Paper

Inhibition of intestinal epithelial apoptosis and survival in a murine model of pneumonia-induced sepsis

JAMA : the Journal of the American Medical Association
Craig M CoopersmithR S Hotchkiss

Abstract

Increased intestinal epithelial apoptosis is present in both human autopsy studies and animal models of sepsis. Whether altering gut apoptosis decreases mortality in sepsis induced by pathogenic bacteria outside the gut is unknown. To determine if decreasing levels of intestinal cell death improves survival in a murine model of Pseudomonas aeruginosa pneumonia-induced sepsis. Prospective study in which transgenic mice that overexpress the antiapoptotic protein Bcl-2 in their intestinal epithelium (n = 25) and control littermates (n = 26) were subjected to intratracheal injection of P aeruginosa. Survival at 7 postoperative days, compared between the 2 groups. Secondary outcomes included quantification of gut epithelial apoptosis. Survival in transgenic mice that overexpress Bcl-2 in the intestinal epithelium was 40% (10/25) compared with 4% (1/26) in control littermates 7 days after intratracheal injection of P aeruginosa (P =.001), with differences in survival noted within 24 hours of surgery. Overexpression of Bcl-2 was associated with a decrease in gut epithelial apoptosis demonstrated by active caspase 3 staining, the terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling assay, and hematoxylin-eosin staining....Continue Reading

Citations

Sep 24, 2013·Trends in Molecular Medicine·Rohit Mittal, Craig M Coopersmith
May 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Richard S HotchkissIrene E Karl
Feb 4, 2014·Trends in Molecular Medicine·Noelle A HutchinsAlfred Ayala
Apr 7, 2011·Critical Care : the Official Journal of the Critical Care Forum·Femke StephanSacha Zeerleder
Jun 10, 2009·Critical Care : the Official Journal of the Critical Care Forum·Jacco J de HaanWim A Buurman
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Aug 31, 2004·Journal of Clinical Pathology·D H WyllieT E A Peto
Jun 1, 2005·The Journal of Surgical Research·Pardis JavadiCraig M Coopersmith
Jun 29, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Joel G R WeaverAndrew D Badley
Sep 13, 2006·Infection and Immunity·Jinghua JiaShouguang Jin
Nov 1, 2007·Respiratory Research·Emanuela Mazzon, Salvatore Cuzzocrea
Feb 29, 2008·American Journal of Physiology. Renal Physiology·Hideo YasudaRobert A Star
Oct 18, 2008·Critical Care : the Official Journal of the Critical Care Forum·Stefan U WeberFrank Stüber
Jan 23, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paul E StrombergCraig M Coopersmith
Feb 18, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·M P Garin-LaflamM B Cohen
Apr 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xin HuangAlfred Ayala
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