Inhibition of intimal hyperplasia by direct thrombin inhibitors in an animal vein bypass model

Annals of Vascular Surgery
Leila MureebeD Silver

Abstract

Many functions of the coagulation system have nonthrombotic effects. The indirect thrombin inhibitor heparin has been previously shown to be effective in limiting intimal hyperplasia (IH). We sought to study the effect of thrombin on IH by using two direct thrombin inhibitors (DTIs), argatroban and lepirudin. Sprague-Dawley rats underwent interposition vein grafting to the carotid artery. Vein grafts were treated with either saline (n = 6) or one of the two DTIs (n = 6 for both). At 30 days, the rats were sacrificed and vessels were perfusion fixed. Sections of the proximal carotid artery, graft, and both anastomoses were stained with both hematoxlyin/eosin and von Gieson's elastin stain. Sections were examined and compared for luminal area and intima-to-media (IM) ratio. The vessels treated with DTIs had less (p < 0.05) IH (IM ratio for proximal anastomosis: control 1.036 +/- 0.857, lepirudin 0.373 +/- 0.21, argatroban 0.182 +/- 0.118) and better lumen preservation than the control vessels (lumen area of proximal anastomosis: control 1.69 +/- 0.9, lepirudin 2.45 +/- 0.74, argatroban 2.81 +/- 0.78). There were no thromboses in the DTI-treated vessels. Dilatation of the graft segment was noted in the argatroban group. Thus, DTIs...Continue Reading

References

Nov 18, 1997·Clinical and Experimental Pharmacology & Physiology·T ImanishiI Nishio
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A Lovich, E R Edelman
Nov 13, 1999·Journal of Vascular Research·G G BishopI J Sarembock
Feb 16, 2000·Thrombosis Research·A Hijikata-OkunomiyaY Ishikawa
Jun 8, 2001·Circulation Research·C PattersonM S Runge
Mar 22, 2002·Journal of Cardiovascular Pharmacology and Therapeutics·B M SasseenR L Wilensky

❮ Previous
Next ❯

Citations

Jun 26, 2007·The Annals of Thoracic Surgery·Edward J W WallittPhilip I Hornick
Nov 2, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sandip SarkarAlexander M Seifalian
Oct 18, 2011·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·D M ScharnJ A van der Vliet
Jul 11, 2006·Journal of Vascular Surgery·Lucy WalesJanet T Powell
May 1, 2007·Current Therapeutic Research, Clinical and Experimental·Shinsuke MiiKiyoshi Tanaka
Sep 13, 2012·Cardiology in Review·Francis Y KimDavid J Whellan
Nov 22, 2014·Plastic and Reconstructive Surgery·Stephanie D MalliarisJeffrey A Ascherman
Dec 7, 2007·Vascular·Leila Mureebe

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.