Inhibition of intrafollicular PGE2 synthesis and ovulation following ultrasound-mediated intrafollicular injection of the selective cyclooxygenase-2 inhibitor NS-398 in cattle

Journal of Animal Science
M W PetersG W Smith

Abstract

Ultrasound-mediated intrafollicular injection and aspiration procedures were used to investigate the ability of the selective cyclooxygenase-2 inhibitor, NS-398, to inhibit intrafollicular PGE2 synthesis and suppress ovulation in dairy cattle. Follicular growth and timing of the preovulatory gonadotropin surge were synchronized in 55 Holstein cows and the position of the ovulatory follicle was determined by daily ultrasound scanning. Preovulatory follicular fluid was aspirated from the largest follicle in four animals at 0, 6, 12, 18, and 24 h after GnRH injection (n = 20). The remaining 35 animals were subjected to ultrasound-mediated intrafollicular injection of NS-398 (10 microM final concentration; n = 19) or diluent (n = 16; controls). At 24 h after GnRH injection, follicular fluid was harvested from a subset of NS-398- (n = 9) and diluent-treated animals (n = 6). The remaining NS-398- and diluent-treated animals were subjected to ultrasonography every 6 h for 36 h after intrafollicular injection, and then daily through d 7 of the subsequent luteal phase to monitor ovulation and corpus luteum development. Follicular fluid PGE2 concentrations were increased following GnRH injection and reached a maximum at 24 h (P < 0.05). ...Continue Reading

References

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Citations

Apr 9, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Brandy L DozierDiane M Duffy
Oct 30, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Rogério FerreiraPaulo Bayard Gonçalves
Mar 11, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·M ZachutU Moallem
May 13, 2014·Animal Biotechnology·Miguel A VelazquezHeiner Niemann
Oct 18, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Ketan ShresthaRina Meidan
Sep 21, 2016·Reproduction in Domestic Animals = Zuchthygiene·E SinderewiczI Woclawek-Potocka
Jan 19, 2018·International Journal of Molecular Sciences·Eva Nagyova
Oct 17, 2007·The Journal of Reproduction and Development·Shu-ichi KobayashiMasashi Takahashi
Nov 11, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Qinglei LiGeorge W Smith
May 31, 2015·Human Reproduction Update·Diane M Duffy
Sep 26, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Soon Ok KimDiane M Duffy
Jan 28, 2020·The Journal of Reproduction and Development·Rebecca LappAndreas Vernunft
Aug 8, 2018·Endocrinology·Ketan Shrestha, Rina Meidan
Dec 30, 2014·Endocrine Reviews·Katja HummitzschRaymond J Rodgers
Nov 19, 2005·The Journal of Reproduction and Development·Ken-Go HayashiAkio Miyamoto
Nov 20, 2020·Animal Cells and Systems·Patric S LundbergDiane M Duffy
Dec 4, 2012·Theriogenology·S N SchauerF X Donadeu

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