PMID: 7536813May 1, 1995Paper

Inhibition of kinesin synthesis in vivo inhibits the rapid transport of representative proteins for three transport vesicle classes into the axon

Journal of Neurochemistry
A AmaratungaR E Fine

Abstract

We have previously demonstrated that the in vivo vitreal injection of an antisense oligonucleotide directed to the kinesin heavy chain inhibits retinal kinesin synthesis by 82% and concomitantly inhibits rapid transport of total protein into the optic nerve by 70%. These results establish a major role for kinesin in rapid axonal transport in vivo. Recently, the cloning of a family of kinesin-like molecules from the mammalian brain has been reported, and some of these proteins are also expressed in neurons. To assign to specific function to the kinesin heavy chain we inhibited the kinesin synthesis with an antisense kinesin oligonucleotide and assessed the axonal transport into the optic nerve of representative proteins from each of three vesicle classes that contain rapidly transported proteins. Marker proteins used were substance P for peptide-containing synaptic vesicles, the amyloid protein for plasma membrane precursor vesicles, and several integral synaptic vesicle proteins. Our results indicate that the major anterograde motor protein for all three vesicle classes utilizes kinesin heavy chain, although we discuss alternative explanations.

Citations

Oct 1, 2011·Experimental Brain Research·Silke BrunholzGerardo Morfini
Dec 28, 1999·Annual Review of Cell and Developmental Biology·L S Goldstein, A V Philp
May 7, 1999·European Journal of Biochemistry·M P Sheetz
Aug 30, 2008·Journal of Cellular Physiology·Yong Zhang, Ji Wu
Mar 9, 2012·Molecular Biology of the Cell·Gerald F ReisLawrence S B Goldstein
Jun 9, 2000·Annual Review of Neuroscience·L S Goldstein, Z Yang
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Nov 10, 2013·Human Molecular Genetics·Christine VillegasZoia Ladescu Muresan
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