PMID: 8609173Apr 1, 1996Paper

Inhibition of laminin alpha 1-chain expression leads to alteration of basement membrane assembly and cell differentiation

The Journal of Cell Biology
A De ArcangelisP Simon-Assmann

Abstract

The expression of the constituent alpha 1 chain of laminin-1, a major component of basement membranes, is markedly regulated during development and differentiation. We have designed an antisense RNA strategy to analyze the direct involvement of the alpha 1 chain in laminin assembly, basement membrane formation, and cell differentiation. We report that the absence of alpha 1-chain expression, resulting from the stable transfection of the human colonic cancer Caco2 cells with an eukaryotic expression vector comprising a cDNA fragment of the alpha 1 chain inserted in an antisense orientation, led to (a) an incorrect secretion of the two other constituent chains of laminin-1, the beta 1/gamma 1 chains, (b) the lack of basement membrane assembly when Caco2-deficient cells were cultured on top of fibroblasts, assessed by the absence of collagen IV and nidogen deposition, and (c) changes in the structural polarity of cells accompanied by the inhibition of an apical digestive enzyme, sucrase-isomaltase. The results demonstrate that the alpha 1 chain is required for secretion of laminin-1 and for the assembly of basement membrane network. Furthermore, expression of the laminin alpha 1-chain gene may be a regulatory element in determinin...Continue Reading

References

Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·M Paulsson
Oct 28, 1992·Annals of the New York Academy of Sciences·K G LawlorR Narayanan
Oct 1, 1992·Current Opinion in Cell Biology·D F MosherJ A McDonald
Jun 1, 1991·Matrix : Collagen and Related Research·T HaaparantaE Engvall
Oct 1, 1990·The Journal of Cell Biology·A Le BivicE Rodriguez-Boulan
Jun 1, 1990·Cell·E R Fearon, B Vogelstein
May 2, 1988·European Journal of Biochemistry·L HartlR Deutzmann
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S RobineD Louvard
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Dec 15, 1983·European Journal of Biochemistry·R TimplG Wick
Apr 1, 1980·Developmental Biology·I LeivoJ Wartiovaara
Sep 29, 1995·Experientia·P Simon-AssmannP Simo
May 1, 1995·The Journal of Cell Biology·C H StreuliM J Bissell
Aug 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·R Timpl, J C Brown
Oct 1, 1994·Current Opinion in Cell Biology·J HülskenW Birchmeier
Jul 15, 1994·European Journal of Biochemistry·L SorokinR Deutzmann
Mar 1, 1994·The Journal of Cell Biology·M L Matter, G W Laurie
Jan 1, 1993·Developmental Genetics·R P Erickson

❮ Previous
Next ❯

Citations

Jul 22, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y KadoyaY Yamada
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
Nov 10, 2000·International Journal of Cancer. Journal International Du Cancer·I SordatB Sordat
Oct 23, 2001·International Journal of Cancer. Journal International Du Cancer·A De ArcangelisP Simon-Assmann
Sep 29, 1995·Experientia·P Simon-AssmannP Simo
Aug 19, 2003·Developmental Biology·Anne Laure Bolcato-BelleminPatricia Simon-Assmann
Apr 28, 2001·Molecular and Cellular Endocrinology·F KuchenbauerM Páez-Pereda
Sep 16, 2003·Molecular and Cellular Endocrinology·F KuchenbauerM Páez-Pereda
Apr 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Peter EkblomJan F Talts
Dec 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·M DurbeejM Ekblom
Dec 23, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M FalkP Ekblom
Oct 1, 1996·Current Opinion in Cell Biology·R Timpl
Oct 1, 1996·Current Opinion in Cell Biology·P Ekblom
Dec 1, 1996·Neuromuscular Disorders : NMD·U M Wewer, E Engvall
Feb 27, 2003·Progress in Retinal and Eye Research·Katsuyoshi SuzukiTeruo Nishida
Mar 16, 2004·Pediatric Diabetes·Rohan K HumphreyAlberto Hayek
Jan 6, 2010·The Journal of Biological Chemistry·Malia M EdwardsPatsy M Nishina
Nov 25, 2003·The Journal of Biological Chemistry·Silvia A PiccinniOlivier Lefebvre
Nov 14, 2008·The Journal of Biological Chemistry·Matthew A SandersMarc D Basson
Dec 11, 1999·The Laryngoscope·D G WeinbergerM Baethmann
Jul 31, 1998·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M KedingerP Simon-Assmann
Feb 3, 1999·Annals of the New York Academy of Sciences·M KedingerJ N Freund
Jan 26, 2002·Molecular and Cellular Biology·Jill ThybollKarl Tryggvason
Jul 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D WarburtonK Anderson
Jul 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J N FreundI Duluc
Apr 7, 2010·The Journal of Clinical Investigation·Georg MellitzerGérard Gradwohl
Feb 15, 2012·Journal of Cell Science·Anna K TownleyDavid J Stephens
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·P D YurchencoJ J O'Rear
Feb 3, 1999·Annals of the New York Academy of Sciences·P Simon-AssmannA De Arcangelis
Apr 18, 1998·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·F C BronfmanN C Inestrosa
Aug 19, 2014·ACS Applied Materials & Interfaces·Alexandre J S RibeiroBeth L Pruitt
Jan 13, 2009·Clinical Biochemistry·Elena KostidouMartha Kaloyianni
Dec 24, 2005·The Journal of Investigative Dermatology·Jie LiM Peter Marinkovich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.