PMID: 6161004Nov 1, 1980Paper

Inhibition of lipid-linked mannose and mannoprotein synthesis in yeast by diumycin in vitro

European Journal of Biochemistry
P Babczinski

Abstract

Diumycin, a phosphoglycolipid antibiotic, inhibits different mannosyl transfer reactions in yeast. Using membrane preparations, the drug effectively inhibited the formation of dolichyl phosphate mannose (DolP-Man); 50% inhibition was observed at approximately 10 microgram/ml. To a lesser extent also mannosyl transfer from DolP-Man to protein decreased in presence of diumycin. Both mannosyl transfer to protein-serine/threonine acceptor sites as well as into positions within the asparagine-linked polymannose part of the yeast mannoprotein are inhibited to about 60% under conditions where DolP-Man formation is blocked. DolP-Man synthesis as well as mannosyl transfer from DolP-Man to protein are also inhibited by diumycin using solubilized enzymes and exogenous acceptor substrates. Glycosyltransfer reactions from GDP-mannose either to protein-serine/threonine-linked mannose (formation of short manno-oligosaccharides) or to dolichyl-diphosphate-linked chitobiose (formation of lipid-linked trisaccharide) are not inhibited by diumycin under conditions where DolP-Man synthesis is blocked by the antibiotic. The inhibitory action of diumycin on DolP-Man formation does not seem to be competitive with respect to dolichyl phosphate, since i...Continue Reading

References

Feb 1, 1978·European Journal of Biochemistry·A J Parodi
Jan 1, 1976·Advances in Microbial Physiology·C Ballou
Aug 13, 1975·Biochimica Et Biophysica Acta·L Lehle, W Tanner
Oct 1, 1973·Biochemical and Biophysical Research Communications·P Babczinski, W Tanner
May 20, 1974·Biochimica Et Biophysica Acta·L Lehle, W Tanner
Jul 1, 1974·European Journal of Biochemistry·C B SharmaW Tanner
Jun 1, 1970·Journal of Pharmaceutical Sciences·J KirschbaumF L Weisenborn
Sep 1, 1968·The Biochemical Journal·R Sentandreu, D H Northcote

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