Inhibition of lipogenesis and induction of apoptosis by valproic acid in prostate cancer cells via the C/EBPα/SREBP-1 pathway

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Bo PangLing Qiao

Abstract

Background: Lipid metabolism reprogramming is now accepted as a new hallmark of cancer. Hence, targeting the lipogenesis pathway may be a potential avenue for cancer treatment. Valproic acid (VPA) emerges as a promising drug for cancer therapy, however, the underlying mechanisms are not yet fully understood. This study aimed to investigate the effects and mechanisms of VPA on cell viability, lipogenesis, and apoptosis in human prostate cancer PC-3 cells.Methods: The effects of VPA on the viability of PC-3 cells were investigated using MTT cell viability assay and wound-healing assay. Oil-Red O staining was used to examine lipid droplets, and DAPI staining assay and Annexin V-FITC and PI double-staining assay were used to measure the extent of cell apoptosis. Quantitative real-time PCR and Western blotting were used to determine the expression of lipogenesis and apoptosis genes. Statistical and analytical data were analyzed with SPSS 17.0 Software, and statistical significance was set to *P < 0.05, **P < 0.01, and ***P < 0.001 levels.Results: The results showed that VPA significantly reduced lipid accumulation and induced apoptosis of PC-3 cells. Moreover, the expression of CCAAT/enhancer-binding protein α (C/EBPα), as ...Continue Reading

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