Inhibition of lipopolysaccharide-induced in vitro desensitization by interferon-gamma

European Journal of Immunology
J G HaasH W Ziegler-Heitbrock

Abstract

Exposure of Mono-Mac-6 cells to lipopolysaccharide (LPS) can induce rapid and transient expression of cytokines like tumor necrosis factor (TNF), interleukin 1 and interleukin 6. Preculture of Mono-Mac-6 cells in culture medium containing small amounts (1-50 ng/ml) of LPS for 3 days leads to an unresponsiveness to a subsequent stimulation with a high amount of LPS. This in vitro desensitization of a monocytic cell line may serve as a model for desensitization to LPS seen in vivo, for example in mice or man repetitively treated with LPS. Addition of interferon-gamma (IFN-gamma) to the Mono-Mac-6 cells during the LPS preculture period leads to an inhibition of desensitization, whereas addition of IFN-alpha or IFN-beta is not able to inhibit the LPS-induced desensitization. The inhibition of desensitization by IFN-gamma was dose dependent and time dependent. Preculture of Mono-Mac-6 cells with LPS leads to a strong reduction of TNF mRNA. This reduction of specific mRNA is also overcome by addition of IFN-gamma, but not by IFN-alpha and IFN-beta, indicating that pretranslational mechanisms are responsible for the regulation of TNF in desensitization.

References

Jul 1, 1989·Journal of Leukocyte Biology·J G HaasH W Ziegler-Heitbrock
Feb 1, 1987·Journal of Interferon Research·F C Nichols, S W Garrison
Mar 15, 1988·International Journal of Cancer. Journal International Du Cancer·H W Ziegler-HeitbrockG Riethmüller
Jun 1, 1986·The Journal of Experimental Medicine·C A DinarelloJ V O'Connor
Oct 24, 1986·Science·K J TraceyJ D Albert
Jun 30, 1947·The Journal of Experimental Medicine·P B Beeson, UNKNOWN Technical Assistance of Elizabeth Roberts

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Citations

Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Janice Chen, Lionel B Ivashkiv
Sep 1, 1996·The Journal of Trauma·M KeelW Ertel
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aideen Byrne, Denis J Reen
Oct 31, 2020·Frontiers in Immunology·Mohammed GhiboubWouter J de Jonge

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