Abstract
Radiotherapy is the mainstay in the treatment of prostate cancer. However, significant radioresistance of castration-resistant prostate cancer (CRPC) cells constitutes a main obstacle in the treatment of this disease. By using bioinformatic data mining methods, LOXL2 was found to be upregulated in both androgen-independent prostate cancer cell lines and radioresistant tumor samples collected from patients with prostate cancer. We speculate that LOXL2 may play an important role in the radioresistance of CRPC cells. The effect of LOXL2 knockdown on the radiosensitivity of androgen-independent prostate cancer cells lines was measured by the clonogenic assay and xenograft tumor experiments under in vitro and in vivo conditions, respectively. In studies on the mechanism, we focused on the EMT phenotype changes and cell apoptosis changes induced by LOXL2 knockdown in DU145 cells. The protein levels of three EMT biomarkers, namely, E-cadherin, vimentin, and N-cadherin, were measured by western blotting and immunohistochemical staining. Cell apoptosis after irradiation was measured by flow cytometry and caspase-3 activity assay. Salvage experiment was also conducted to confirm the possible role of EMT in the radiosensitization effect o...Continue Reading
References
Jun 19, 2008·Cancer Research·Héctor PeinadoAmparo Cano
Dec 23, 2009·The Journal of Biological Chemistry·Ruth SchietkeMichael S Wiesener
Mar 23, 2010·Molecular Biology Reports·Young-Mi KimYoungho Kim
Jun 18, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jan TheysMarc Vooijs
Oct 3, 2012·Biochemical and Biophysical Research Communications·Hongliang YuDemin Zhou
Aug 13, 2013·Breast Cancer Research and Treatment·Sung Gwe AhnJeffrey E Green
Mar 19, 2014·Oncogene·G CanesinA Cano
Aug 17, 2014·Cancer Letters·Hiroaki KasashimaKosei Hirakawa
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Sep 1, 2015·International Journal of Oncology·Xi LiangJianming He
Jun 10, 2016·Journal of Human Genetics·Mayuko KatoNaohiko Seki
Jun 21, 2016·International Journal of Radiation Oncology, Biology, Physics·Kamran A AhmedJavier F Torres-Roca
Sep 15, 2016·The New England Journal of Medicine·Freddie C HamdyUNKNOWN ProtecT Study Group
Oct 4, 2016·Oncogene·D H PengD L Gibbons
Sep 4, 2017·Seminars in Radiation Oncology·Mélanie L FerlazzoNicolas Foray
Oct 25, 2017·The Prostate·Timothy W StarkNatasha Kyprianou
Mar 23, 2018·International Journal of Molecular Sciences·Cameron A Wade, Natasha Kyprianou
Mar 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Xi ZhangMinhao Liu
Citations
Sep 25, 2019·Cancers·Julia SmentochNatalia Bednarz-Knoll
May 17, 2019·Journal of Clinical Medicine·Patricia G SantamaríaAmparo Cano
Mar 3, 2020·Frontiers in Molecular Biosciences·Erik HenkeSüleyman Ergün
Aug 26, 2020·Expert Opinion on Drug Delivery·Sara GouarderesLaure Gibot
Apr 4, 2021·Cancers·Desirée Martínez-MartínezMarina Lasa
Sep 26, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Bing WenEn-Min Li
Jul 3, 2021·Cancers·Sofia PapanikolaouKostis Gyftopoulos
Jul 25, 2021·Cancers·Ismahane BelhabibChristine Jean