PMID: 6170333Oct 2, 1981Paper

Inhibition of lymphocyte activation by ouabain. Interference with the early activation of membrane phospholipid metabolism

Biochimica Et Biophysica Acta
M Szamel, K Resch

Abstract

Activation of lymphocytes by antigens and mitogens can effectively be prevented by ouabain, a known inhibitor of (Na+ + K+)-ATPase. Recently it was shown that lowering of intracellular levels of monovalent cations is not involved in the inhibitory effect of ouabain. (Na+ + K+)-ATPase was found to be closely associated with acyl-CoA : lysophosphatidylcholine acyltransferase in the plasma membrane of lymphocytes. Both enzymes are activated as an immediate consequence of mitogen binding. Human peripheral lymphocytes were stimulated with concanavalin A. Ouabain suppressed the induction of RNA and DNA synthesis in a concentration-dependent way. Increase of RNA synthesis was suppressed only if the glycoside were added within the first hours of activation. If ouabain was added later, incorporation of uridine remained at the rate that was reached at the time of glycoside administration, pointing to an early event where ouabain may be operative. Ouabain, in a dose-dependent manner similar to that affecting RNA and DNA synthesis, inhibited the increase in the incorporation of oleate into phospholipids in stimulated lymphocytes, whereas the turnover of phospholipid fatty acids in resting lymphocytes was unaffected. Increasing extracellula...Continue Reading

References

Nov 1, 1970·Experimental Cell Research·M R Quastel, J G Kaplan

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Citations

Oct 12, 2012·Parasitology Research·P L JacobS Rodrigues-Mascarenhas
May 25, 2002·European Journal of Biochemistry·Zijian Xie, Amir Askari
Mar 1, 1986·Archives of Biochemistry and Biophysics·L PajorT Farkas
Jun 17, 2015·Mediators of Inflammation·Jacqueline Alves LeiteSandra Rodrigues-Mascarenhas
Nov 1, 1995·Journal of Cellular Physiology·C BrodieE W Gelfand
Jun 9, 2000·Annual Review of Physiology·A J Hulbert, P L Else

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