Inhibition of lymphocyte mitogenesis by an arachidonic acid hydroperoxide

Journal of Supramolecular Structure
M G Goodman, W O Weigle

Abstract

Incubation of murine spleen cells with the oxidation product of soybean lipoxidase-treated arachidonic acid results in profound inhibition of induction of proliferation and maturation of these cells. The active entity was shown to be the 15-hydroperoxide of arachidonic acid (15-HPAA). Inhibition of the enzymes of the cyclo-oxygenase pathway fails to disturb this effect, indicating that 15-HPAA is not a substrate for this series of enzymes. 15-HPAA produced in this manner interfered with RNA synthesis, DNA synthesis, and blastogenesis, while failing to exert cytotoxic effects on the cells themselves. A variety of lymphocyte subpopulations, distinguished by their responsiveness to a diverse group of mitogens, were all equally inhibited by the addition of 15-HPAA to culture. Addition of this agent even as late as 24 h after initiation of culture resulted in profound inhibition of the proliferative and differentiative responses of splenic B cells to bacterial lipopolysaccharide (LPS). Exposure of cells to 15-HPAA for 10-30 min was adequate to initiate inhibition, an event that exhibited marked temperature dependence. The effects of pre-incubation with 15-HPAA could not be reversed in its absence in recovery periods of up to 6 h pri...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H Hidaka, T Asano
Mar 1, 1978·Prostaglandins·J A Salmon
Aug 1, 1974·European Journal of Immunology·J Andersson, F Melchers
Sep 1, 1967·The Journal of Experimental Medicine·R I Mishell, R W Dutton
Apr 26, 1963·Science·N K Jerne, A A Nordin

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Citations

Jan 1, 1981·Immunological Reviews·P Y PatersonC C Whitacre
Jan 1, 1988·Progress in Lipid Research·A A SpectorS A Moore

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