Oct 1, 1975

Inhibition of macrophage migration by virus-induced interferon preparations

Japanese Journal of Microbiology
J Imanishi

Abstract

It was found that a preparation of mouse L cell interferon induced by Newcastle disease virus (NDV) possessed not only interferon activity but also inhibitory activity upon migration of guinea pig peritoneal macrophages (MIF activity). These activities were also observed in a preparation of human leukocyte interferon induced by NDV. The interferon and MIF activities shared common characteristics in the dose response, time course of in vitro production, thermal stability, sensitivity to trypsin and periodate, and elution pattern in CM-Sephadex column chromatography. However, gel filtration pattern with Sephadex G-100 showed two separate peaks. Fractions collected from the first peak, corresponding to a molecular weight of about 45 000, had only the MIF activity, while those collected from the second peak, corresponding to a molecular weight of about 30 000, had both the interferon and MIF activities. A preparation of mouse brain interferon induced by Japanese encephalitis virus had a much weaker MIF activity than the L cell interferon, although these preparations were equal in interferon activity (5000 units/ml).

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Mentioned in this Paper

Macrophages, Peritoneal
Newcastle disease virus
Cavia
Brain
Human leukocyte interferon
Encephalitis Viruses
Interferon
Trypsin
Japanese Encephalitis
Leukocytes

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