Inhibition of malonaldehyde formation in oxidized calf thymus DNA with synthetic and natural antioxidants

Journal of Agricultural and Food Chemistry
Hiroshi Matsufuji, Takayuki Shibamoto

Abstract

Calf thymus DNA was oxidized by Fenton's reagent with or without synthetic antioxidants (Trolox and DMPO) and natural antioxidants-quercetin, apigenin, 2''-O-glycosylisovitexin (2''-O-GIV), (+)-catechin, cyanidin, pelargonidin, keracyanin, and callistephin. Malonaldehyde (MA) formed in oxidized DNA was analyzed using gas chromatography. MA formed from oxidized DNA without antioxidants was 4.0 +/- 0.53 nmol/mg of DNA in buffer solution, 3.7 +/- 0.34 nmol/mg of DNA in NaOH solution, and 4.6 +/- 0.19 nmol/mg of DNA in HCl solution. MA formed from DNA with antioxidants (at the level of 0.1 micromol/mL) ranged from 1.90 +/- 0.18 (catechin) to 4.10 +/- 0.18 nmol/mg of DNA (cyanidin). Trolox and DMPO inhibited MA formation from DNA by 41.2% and 18.6%, respectively, at the level of 0.1 micromol/mL. Trolox (water-soluble vitamin E) exhibited dose-dependent inhibition. The decreasing order of inhibitory effect by flavonoids at the level of 0.1 micromol/mL was catechin (48.5%) > quercetin (47.1%) > 2''-O-GIV (40.5%) > apigenin (29.9%) and by the anthocyanins at the level of 0.1 micromol/mL was callistephin (45%) > keracyanin (33.2%) > pelargonidin (25.1%) > cyanidin (10.2%).

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Citations

Feb 25, 2006·Journal of Pharmaceutical and Biomedical Analysis·T Shibamoto
May 26, 2016·Phytotherapy Research : PTR·Antonella SmeriglioDomenico Trombetta
Oct 13, 2017·Nutrients·Tarun BelwalSolomon Habtemariam
Jun 21, 2007·Molecular Nutrition & Food Research·Suresh K AbrahamHelga Stopper
Feb 3, 2009·Journal of Agricultural and Food Chemistry·Joon-Kwan Moon, Takayuki Shibamoto

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