Inhibition of measles virus and subacute sclerosing panencephalitis virus by RNA interference

Antiviral Research
Momoko OtakiHak Hotta

Abstract

Subacute sclerosing panencephalitis (SSPE) is a rare, but fatal outcome of measles virus (MeV) infection. SSPE develops after prolonged persistence of mutated MeV called SSPE virus. Although a combination therapy using interferon and inosiplex or ribavirin appears to prolong survival time to some extent, there is currently no effective treatment to completely cure SSPE and a new treatment strategy is greatly needed. In this study, we adopted RNA interference (RNAi) strategy and examined whether small interfering RNAs (siRNAs) can be used to inhibit replication of MeV and SSPE virus. We report here that siRNAs targeted against L mRNA of MeV, either synthetic siRNAs or those generated by pcPUR+U6i-based expression plasmids, effectively and specifically inhibited replication of both MeV and SSPE virus without exhibiting any cytotoxic effect. The L protein of MeV is a major component of RNA-dependent RNA polymerase that is essential for viral RNA replication, and yet it is least abundant among all the MeV proteins expressed. Therefore, mRNA encoding the L protein would be a good target for RNAi strategy. The present results imply the possibility that our siRNAs against MeV L mRNA are among the potential candidates to be used to tre...Continue Reading

References

Mar 1, 1991·The Journal of General Virology·F KobuneA Sugiura
Sep 1, 1989·International Journal of Epidemiology·Y OkunoI Kitamura
Apr 18, 2002·Genes & Development·Patrick J PaddisonDouglas S Conklin
May 1, 2002·Nature Biotechnology·Cynthia P PaulDavid R Engelke
Jun 28, 2002·Nature·Jean-Marc JacqueMario Stevenson
Jul 5, 2002·Nature·Anton P McCaffreyMark A Kay
Sep 24, 2002·Nature Biotechnology·Haibin XiaBeverly L Davidson
Aug 28, 2003·Nature Cell Biology·Carol A SledzBryan R G Williams
Oct 15, 2003·Journal of Virology·Daniel BodenBharat Ramratnam
Jan 23, 2004·Journal of Child Neurology·Generoso G Gascon, UNKNOWN International Consortium on Subacute Sclerosing Panencephalitis
Feb 14, 2004·Journal of Virology·Atze T DasBen Berkhout
Mar 3, 2004·Nature Biotechnology·Dong-Ho KimJohn J Rossi
Apr 8, 2004·Virus Research·Glenn Randall, Charles M Rice
Jun 10, 2004·Antiviral Chemistry & Chemotherapy·Hiroshi Takaku
Jun 15, 2004·Virology·Senthil K RadhakrishnanAndrei L Gartel
Dec 28, 2004·Nature Medicine·Vira BitkoSailen Barik
Jan 18, 2005·Antiviral Research·Chang-Jer WuYi-Lin Chan
Jul 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dhruba J BharaliMichal K Stachowiak
Nov 18, 2005·Antiviral Research·Peter M HuelsmannKarin J Metzner

❮ Previous
Next ❯

Citations

Apr 15, 2010·Medical Microbiology and Immunology·Dajana Reuter, Jürgen Schneider-Schaulies
Jul 10, 2009·Journal of Virology·Michael ZinkeJürgen Schneider-Schaulies
Feb 5, 2010·Canadian Journal of Microbiology·Jun-Yan ShiEn-Jie Luo
Apr 10, 2007·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Banu Anlar
Jun 22, 2010·Developmental Medicine and Child Neurology·Jose GutierrezBarbara S Koppel
Jan 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Patrick KetzerDirk M Nettelbeck
Apr 19, 2015·Virology Journal·Alaa M H El-BitarHak Hotta
Apr 16, 2018·Reviews in Medical Virology·Abid QureshiAbdul Ghani Ahangar
Mar 28, 2012·Expert Review of Neurotherapeutics·Burak TatliMeral Ozmen
Nov 7, 2019·Viruses·Marion FerrenCyrille Mathieu
Nov 21, 2020·BMC Veterinary Research·Otávio Valério de CarvalhoAbelardo Silva-Júnior

❮ Previous
Next ❯

Datasets Mentioned

BETA
AB016162

Methods Mentioned

BETA
transfection

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.