PMID: 9541646Apr 29, 1998Paper

Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells

Mutation Research
C KeshavaT M Ong

Abstract

Methotrexate (MTX), an anticancer compound, is widely used in the treatment of leukemia. It induces cytogenetic damage as well as cytostatic effects on a variety of cell systems. Folinic acid (Leucovorin) is generally administered along with MTX as a rescue agent to decrease MTX-induced toxicity. However, information regarding the inhibitory effect of folinic acid against cytogenetic damage caused by MTX is limited. This study was conducted to assess the cytogenetic effect of MTX and its inhibition by folinic acid (FA) using the micronucleus and chromosomal aberration assays concurrently. Exponentially growing V79 cells were treated with MTX at five different concentrations (5-100 micrograms ml-1) with S9 microsomal fraction for 6 h and post-treated with two concentrations of FA (5 or 50 micrograms) for 40 h. Results indicate that MTX alone induced a concentration-related increase in % micronucleated binucleated cells (MNBN) and % aberrant cells (Abs). There was a decrease in nuclear division index (NDI) with increase in MTX concentration. Similarly, the mitotic index (MI) also decreased in all concentrations of MTX tested. The addition of FA at 50 micrograms ml-1 significantly reduced % MNBN (40-68%) and % Abs (36-77%). Inhibi...Continue Reading

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Citations

Sep 5, 2002·Teratogenesis, Carcinogenesis, and Mutagenesis·Ciĝdem EgelNilüfer Aydemir
Mar 23, 2005·Mutation Research·Zhanna KirpnickRobert H Schiestl
Aug 5, 2000·Environmental Toxicology and Pharmacology·R C ChoudhuryA K Palo
Nov 7, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robert St OngeMarie Evangelista
Jan 15, 2016·Environmental and Molecular Mutagenesis·Steven M BryceStephen D Dertinger
Aug 30, 2008·Regulatory Toxicology and Pharmacology : RTP·A VikramG B Jena
Mar 2, 2011·Physical Chemistry Chemical Physics : PCCP·Ana Dora Rodrigues PontinhaAna Maria Oliveira-Brett
Apr 4, 2017·Environmental and Molecular Mutagenesis·Steven M BryceStephen D Dertinger
May 14, 2009·Molecular Cancer Research : MCR·Ming ChenSeth J Orlow

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