Inhibition of microRNA-17/20a suppresses cell proliferation in gastric cancer by modulating UBE2C expression

Oncology Reports
Yingyi ZhangYajie Wang

Abstract

microRNAs (miRNAs) are small non-coding RNAs that potentially play a critical role in carcinogenesis. Increasing evidence indicates that the miR-17/20 cluster is upregulated in numerous types of human cancers including gastric cancer which suggests that the miR-17/20 cluster may play an important role in tumorigenesis. However, its role in gastric cancer carcinogenesis remains poorly defined due to the lack of target gene information. The aim of the present study was to investigate the target genes of the miR-17/20 cluster and their role in the tumor growth of gastric cancer. We found that both miR-17 and miR-20a (miR-17/20a) target the UBE2C gene in gastric cancer cells. Luciferase assay, qRT-PCR and western blot analysis confirmed that UBE2C is a direct target of miR‑17/20a in gastric cancer cells. Our results showed that the expression of UBE2C was positively regulated by miR-17/20a at both the mRNA and protein levels. Moreover, miR-17/20a was upregulated and positively associated with UBE2C in the gastric cancer tissues when compared to the adjacent nontumor tissues. Inhibition of miR-17/20a in gastric cancer cells was statistically correlated with a decrease in cell growth. These results demonstrate that upregulation of mi...Continue Reading

References

Mar 10, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G EiriksdottirR B Barkardottir
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·J B WelshG M Hampton
Jun 10, 2005·Nature·Lin HeScott M Hammond
Jun 10, 2005·Nature·Kathryn A O'DonnellJoshua T Mendell
Aug 18, 2005·British Journal of Cancer·P PallanteA Fusco
Jan 28, 2006·World Journal of Gastroenterology : WJG·Ling Yang
Feb 14, 2008·Nature Reviews. Molecular Cell Biology·Giovanni Stefani, Frank J Slack
Aug 13, 2008·The Journal of Cell Biology·Zuoren YuRichard G Pestell
Nov 29, 2008·Cancer Science·Takeo FujitaHiroyoshi Doihara
Feb 24, 2009·Critical Reviews in Oncology/hematology·Vincenzo CatalanoEric Van Cutsem
Mar 7, 2009·The American Journal of Pathology·Rosa Visone, Carlo M Croce
Jul 28, 2009·Nature Cell Biology·Sze Wan ShanBurton B Yang
Jan 13, 2010·The Journal of Cell Biology·Janine H van ReeJan M van Deursen
Apr 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Zuoren YuRichard G Pestell
Apr 23, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Feng MaXuetao Cao
Jun 11, 2010·Annual Review of Biochemistry·Marc Robert FabianWitold Filipowicz
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Jan 24, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Rui XiangQin Zhou
Jun 23, 2012·Digestive Diseases·Alexander LinkPeter Malfertheiner
Apr 9, 2013·The Journal of Allergy and Clinical Immunology·Dihan ZhuKe Zen

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Citations

Apr 18, 2018·PLoS Computational Biology·Marco Del GiudiceCarla Bosia
Aug 4, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·A MeyerA Mokhir
Jul 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Silin ChenXiaodong Liu
Feb 28, 2020·Experimental and Therapeutic Medicine·Zheng XiaoXi Zeng
Jun 4, 2019·Frontiers in Pharmacology·Svetlana Miroshnichenko, Olga Patutina
Sep 28, 2016·World Journal of Gastroenterology : WJG·Kelly Cristina da Silva OliveiraDanielle Queiroz Calcagno
Sep 18, 2015·World Journal of Gastroenterology : WJG·Ya-Kai Huang, Jian-Chun Yu
May 26, 2018·Biological Reviews of the Cambridge Philosophical Society·Shama P KabekkoduKapaettu Satyamoorthy
Mar 27, 2020·Journal of Clinical Laboratory Analysis·Haiyuan SunBo Sun
Jan 6, 2018·Cancer Biology & Therapy·Yan ZhangChang Liu

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