PMID: 3465799Oct 1, 1986

Inhibition of microsomal NAD(P)H oxidation by Triton X-100

Journal of Biochemical and Biophysical Methods
J A Boutin

Abstract

The non-ionic detergent Triton X-100 is shown to inhibit the spontaneous oxidation of NAD(P)H associated with rat liver microsomes. Advantage of this observation is taken to measure different microsomal NAD(P)H-dependent oxidoreductase activities such as 3-alpha-hydroxysteroid dehydrogenase, dihydrodiol dehydrogenase and various xenobiotic oxidoreductases. This inhibition provides an easy method for the screening of the under-investigated microsomal oxidoreductive metabolism of xenobiotics.

Citations

Jan 15, 1977·Archives of Biochemistry and Biophysics·I Jansson, J B Schenkman
Feb 1, 1984·Archives of Biochemistry and Biophysics·M C BarberoJ M Macarulla
Jul 16, 1982·Biochemical and Biophysical Research Communications·O M SinghG C Wood
Jan 1, 1980·Progress in Lipid Research·A A SpectorB T Hyman

Related Concepts

Metabolic Process, Cellular
DHRS9 gene
Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
Isoprenoids
Microsomes, Liver
Oxidation
Xenobiotics
3-Hydroxysteroid Dehydrogenases
Metabolic Pathway
Metabolic Inhibition

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