PMID: 9545554Apr 18, 1998Paper

Inhibition of migration and proliferation of vascular smooth muscle cells by dehydroepiandrosterone sulfate

Biochimica Et Biophysica Acta
Daisuke FurutamaNakaaki Ohsawa

Abstract

Dehydroepiandrosterone (DHEA) and its sulfate (DHEA-S) are the most abundant steroids in humans, and their serum concentrations progressively decrease with age. Although relationships between DHEA(-S) and many age-related illnesses have been postulated, the mechanisms for their effects remain unknown, and specific receptors for these molecules have not been identified. In this paper, to investigate the role of DHEA(-S) in atherogenesis, we studied the proliferation and migration of a rabbit vascular smooth muscle cell line, SM-3, in the presence of DHEA(-S). Cellular proliferation was inhibited by DHEA-S, and to a lesser extent by DHEA. Modified Boyden's chamber assays revealed that DHEA-S inhibited the migration of SM-3 cells toward PDGF-BB. In cell attachment assays, DHEA-S inhibited the attachment of SM3 cells to fibronectin. It was suggested that the inhibitory effect of DHEA-S for SM-3 proliferation and migration was due to the decreased interaction with fibronectin. Scatchard analysis revealed the presence of two populations of DHEA-S binding sites in the nuclear fraction, and a smaller number in the cytosolic fraction. Since the dissociation constant of the higher affinity site was similar to the serum DHEA-S concentrati...Continue Reading

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Citations

Apr 6, 2004·Atherosclerosis·Peter Alexandersen, Claus Christiansen
Aug 29, 2009·Journal of Atherosclerosis and Thrombosis·Tadashi YamakawaYasuo Terauchi
Apr 8, 2011·British Journal of Pharmacology·Chao-Yu Miao, Zhi-Yong Li
Jul 10, 2012·Biochimica Et Biophysica Acta·Cynthia J Koziol-WhiteReynold A Panettieri
Apr 19, 2011·European Journal of Pharmacology·Rebeca López-MarureJoseph S Dillon
Dec 9, 2008·Acta Pharmacologica Sinica·Heng-hui ChengQiu-rong Ruan
Apr 18, 2003·Endocrine Reviews·Fredrick C W Wu, Arnold von Eckardstein
Oct 25, 2002·Cardiovascular Drug Reviews·Wolfgang Weidemann, Hartmut Hanke

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