Inhibition of migration but stimulation of proliferation of human retinal pigment epithelial cells cultured with uniform vesicles of silicone oil.

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Li-Na MaJi-Xian Ma

Abstract

It has been hypothesized that emulsification of silicone oil might stimulate preretinal membrane formation and proliferative vitreoretinopathy. This study aimed to test the effects of vesicles of silicone oil (VSO), which were prepared by a novel membrane emulsification technique, on the migration and proliferation of human retinal pigment epithelial (hRPE) cells in vitro. VSO were produced by extrusion of the oil through a Supor membrane (Pall Life Sciences, Port Washington, NY, USA) with 0.45 microm pore size under a pressure of 0.1 MPa. hRPE cells were incubated with stained 25% VSO for 24 h to assess whether hRPE cells could phagocytize VSO. A wound-healing model was used by denuding cells from a glass slide to assess the migration of hRPE cells. The cells were then incubated with 25%, 50% and 100% VSO with or without serum for up to 72 h. The number of cells that had entered the denuded area was counted under a microscope. To assess the proliferative activity, the cells were incubated with 25%, 50% and 100% VSO, with or without serum, for 24 h. A total of 100 nuclei were examined for each slide, and the numbers of stained argyrophilic nucleolar organizer regions (AgNORs) in the nuclei were measured. The mean vesicle diamet...Continue Reading

References

Jan 1, 1991·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·H P HeidenkummerS Thierfelder
Apr 1, 1987·Archives of Ophthalmology·A CrispJ Tiedeman
Jul 1, 1985·Survey of Ophthalmology·E de JuanJ Tiedeman
Dec 10, 1999·Micron : the International Research and Review Journal for Microscopy·M Derenzini
Apr 5, 2000·Seminars in Ophthalmology·S Yamamoto, S Takeuchi
Aug 10, 2000·International Journal of Legal Medicine·M Rebolledo GodoyM Oehmichen
Feb 1, 2002·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·T AbeM Tamai
Sep 12, 2002·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Yu-Sheng WangPeter Wiedemann
Nov 1, 1962·Archives of Ophthalmology·P A CIBISS CANAAN
Oct 1, 2003·American Journal of Ophthalmology·Juliet Chung, Richard Spaide
May 3, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Norbert KociokAntonia M Joussen
Dec 13, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Margrit HollbornLeon Kohen
Dec 31, 2005·Investigative Ophthalmology & Visual Science·Claudia S AlgeAnselm Kampik
Jun 13, 2006·Japanese Journal of Ophthalmology·Lu-Chun WangChung-May Yang
Dec 28, 2006·European Journal of Ophthalmology·P CarpinetoL Mastropasqua

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Citations

Mar 15, 2015·Journal of Biomaterials Applications·Albert CaramoyRachel L Williams

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