PMID: 9182857Jan 1, 1997Paper

Inhibition of mineralocorticoid activity by the beta-isoform of the human glucocorticoid receptor

The Journal of Steroid Biochemistry and Molecular Biology
C M BambergerH M Schulte

Abstract

Mineralocorticoids and glucocorticoids are important regulators of electrolyte homeostasis and arterial blood pressure. Their effects are mediated by the mineralocorticoid (MR) and the glucocorticoid receptor (GR), respectively. The present study was designed to determine how the two isoforms of the human GR, the "classic" GR alpha and the non-hormone-binding GR beta, interfere with the transcriptional effects of the hormone-activated human MR. COS-7 monkey kidney cells were transfected with different mineralocorticoid-responsive reporter plasmids and a vector expressing the human MR protein. Different amounts of either control, GR alpha, or GR beta plasmid were co-transfected, and luciferase activity was measured after stimulation with aldosterone and/or dexamethasone. MR-mediated stimulation of transcription was enhanced by co-transfection of the GR alpha expression vector. In contrast, MR-mediated stimulation of transcription was strongly inhibited by co-transfection of equal amounts of the GR beta expression vector. Reverse transcription-polymerase chain reaction (RT-PCR) showed expression of both GR isoforms as well as of MR in the human kidney. These data indicate that the two isoforms of the human GR exert opposite effec...Continue Reading

References

Sep 1, 1988·Journal of Neuroscience Research·J A Van EekelenM C Bohn
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·J W Funder
Jun 1, 1995·The Journal of Clinical Investigation·C M BambergerG P Chrousos
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Citations

Jan 23, 1999·Current Opinion in Nephrology and Hypertension·N Farman
Apr 2, 2013·Journal of Neural Transmission·Diego L RovarisClaiton H D Bau
Feb 24, 2001·American Journal of Physiology. Renal Physiology·N Farman, M E Rafestin-Oblin
Aug 10, 2004·Nature Neuroscience·D KauferR M Sapolsky
Aug 14, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S BeavanJ Compston
Nov 24, 1999·The Journal of Clinical Endocrinology and Metabolism·C M BambergerH M Schulte
Mar 7, 2002·American Journal of Physiology. Renal Physiology·James D Stockand
Jul 29, 2009·Nuclear Receptor Signaling·Michiel van der Vaart, Marcel J M Schaaf
Jun 17, 1998·Endocrine Reviews·E R De KloetM Joëls
Aug 23, 2002·Journal of the American Society of Nephrology : JASN·Olivier Bonny, Bernard C Rossier
Mar 24, 1998·Molecular and Cellular Endocrinology·C M BambergerH M Schulte
Feb 7, 2001·Trends in Endocrinology and Metabolism : TEM·A Vottero, G P Chrousos
Dec 24, 1998·Molecular and Cellular Endocrinology·G Piwien-Pilipuk, M D Galigniana
Aug 7, 1999·Progress in Retinal and Eye Research·R J Wordinger, A F Clark
Jul 26, 2002·The Journal of Steroid Biochemistry and Molecular Biology·R H DeRijkE R de Kloet
Mar 15, 2006·Journal of Chemical Neuroanatomy·Anastasia GaleevaMarkku Pelto-Huikko

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