Inhibition of miR-222-3p activity promoted osteogenic differentiation of hBMSCs by regulating Smad5-RUNX2 signal axis

Biochemical and Biophysical Research Communications
Jihong YanDeshan Zhou

Abstract

miRNAs are recently found playing important roles in osteogenesis. In this study, we identified that miR-222-3p decreased during osteogenic differentiation of human mesenchymal stem cells (hBMSCs) using Quantitative Real-Time Reverse Transcription PCR (qRT-PCR). Furthermore, we investigated the effect of miR-222-3p on osteogenic differentiation of hBMSCs. Inhibition of miR-222-3p function in hBMSCs using infection of lentiviruses carrying miR-222-3p specific inhibitor promoted expression of osteoblast-specific genes, alkaline phosphatase (ALP) activity, and matrix mineralization. Whereas, overexpression of miR-222-3p inhibited osteoblast differentiation of hBMSCs in vitro. Moreover, Smad5 and RUNX2, which are the critical transcription factors in osteogenic differentiation, were predicted to be targets of miR-222-3p by bioinformatic analysis. Overexpression of miR-222-3p in hBMSCs significantly suppressed the protein levels of Smad5 and RUNX2, while inhibition of miR-222-3p increased their protein levels. Furthermore, inhibition of miR-222-3p increased phosphorylation of Smad1/5/8, which regulated the expression of osteogenic genes. Our findings suggest that suppression of miR-222-3p activity promoted osteogenic differentiation...Continue Reading

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Citations

Jul 16, 2016·Stem Cells and Development·Claudine SeeligerMartijn van Griensven
Mar 5, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Elodie FeurerRoland Chapurlat
Jul 2, 2019·Molecular Medicine Reports·Daliang KongFei Wang
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Jul 11, 2019·Current Molecular Biology Reports·Benjamin J WildmanQuamarul Hassan
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Hanxiao ZhaoXiaojuan He
Mar 7, 2021·International Journal of Molecular Sciences·Chiara MazziottaFernanda Martini
Jul 10, 2017·ACS Biomaterials Science & Engineering·Eleni K TsekouraHasan Uludag

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