Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB.

Cell Death & Disease
Zuotian HuangZhongjun Wu

Abstract

Hepatic ischemia/reperfusion injury (IRI) is an unavoidable course in liver transplantation, during which the immune response of inflammation plays a leading part. MicroRNA-450b-5p (miR-450b-5p), which has been reported to participate in several inflammatory diseases, was investigated in this study. The purpose of this study is to identify the potential function of miR-450b-5p toward remission of hepatic IRI and elucidate the specific mechanism. Herein we found that expression of miR-450b-5p, interleukin (IL)-1β, tumor necrosis factor-α (TNF-α), and IL-6 was stimulated in hepatic IRI. Inhibition of miR-450b-5p could remarkably alleviate mouse hepatic IRI and improve liver function measured by hematoxylin-eosin (HE) staining, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL), and enzyme-linked immunosorbent assay (ELISA). We further assessed protein expression undergoing Western blot and immunofluorescence, and discovered that miR-450b-5p suppressed alpha B-crystallin (CRYAB), thus restraining the inhibitory κB kinase (IKK) β-mediated canonical nuclear factor-κB (NF-κB) signaling, instead of the noncanonical path guided by IKKα in hepatic IRI. In addition, we demonstrated CRYAB as an activator of M2 polarizati...Continue Reading

References

Nov 19, 2010·Parasitology Research·Denis DelićFrank Wunderlich
Oct 21, 2011·Journal of Gastroenterology and Hepatology·Takashi MasudaSeigo Kitano
Jan 28, 2014·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Ying LuoJia-Hong Dong
Apr 23, 2014·Journal of Hepatology·Andreas RickenbacherPierre-Alain Clavien
Aug 5, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hong ShenLiangyou Rui
Oct 14, 2014·Journal of Hepatology·Simonetta BandieraMirjam B Zeisel
Jan 9, 2015·Archives of Toxicology·Kelly M QuesnelleLuis H Toledo-Pereyra
Feb 18, 2017·Nature Reviews. Drug Discovery·Rajesha Rupaimoole, Frank J Slack
Mar 31, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ziru LiWeizhen Zhang
Jun 25, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chao ZhangWei An
Aug 27, 2017·Journal of Hepatology·Kojiro NakamuraJerzy W Kupiec-Weglinski
Sep 8, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Keely MarshallStephen Tomlinson
Dec 2, 2017·Nature Communications·Hye-Sook HanSeung-Hoi Koo
Nov 9, 2018·Oxidative Medicine and Cellular Longevity·Iman O Sherif, Nora H Al-Shaalan
Jan 29, 2019·Bioscience, Biotechnology, and Biochemistry·Zuotian HuangZhongjun Wu
Mar 5, 2019·Kidney International·Titi ChenDavid C H Harris
May 18, 2019·Cell Death & Disease·Zhe GuoQi Wang
Jun 27, 2019·OncoTargets and Therapy·JunFei ZhangLiShan Yang
Jul 23, 2019·The Journal of Clinical Investigation·Kojiro NakamuraJerzy W Kupiec-Weglinski

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Citations

Nov 24, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fatemeh Sabet SarvestaniAli-Mohammad Tamaddon
Mar 31, 2021·Acta Biochimica Et Biophysica Sinica·Jiangwen DaiZhongjun Wu
Apr 12, 2021·International Immunopharmacology·Yun-Hai LuoZhong-Jun Wu
May 25, 2021·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Jun-Liang PuZhong-Jun Wu
Aug 3, 2021·Molecular and Cellular Biochemistry·Xiaolei GongHaibo Zhang
Jul 24, 2021·Molecular Medicine Reports·Qiwen YuShuijun Zhang

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Methods Mentioned

BETA
nuclear translocation
transfection
confocal
ELISA
Assay

Software Mentioned

Targetscan7
GraphPad Prism
CRYAB

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