Inhibition of miR-9-5p suppresses prostate cancer progress by targeting StarD13

Cellular & Molecular Biology Letters
Lin ChenJiajun Yu

Abstract

This study aims to investigate the effects of inhibiting microRNA-9-5p (miR-9-5p) on the expression of StAR-related lipid transfer domain containing 13 (StarD13) and the progress of prostate cancer. The mRNA expression levels of miR-9-5p and StarD13 were determined in several prostate cancer cell lines. We chose DU145 and PC-3 cells for further research. The CCK8 assay was used to measure the cell viability. The cell invasion and wound-healing assays were respectively applied to evaluate invasion and migration. The expression of E-cadherin (E-cad), N-cadherin (N-cad) and vimentin were measured via western blot. DU145 and PC-3 cells overexpressing StarD13 were generated to investigate the variation in proliferation, invasion and migration. A luciferase reporter assay was used to identify the target of miR-9-5p. Our results show that miR-9-5p was highly expressed and StarD13 was suppressed in prostate cancer cells. MiR-9-5p inhibition repressed the cells' viability, invasion and migration. It also increased the expression of E-cad and decreased that of N-cad and vimentin. StarD13 overexpression gave the same results as silencing of miR-9-5p: suppression of cell proliferation, invasion and migration. The bioinformatics analysis pr...Continue Reading

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Citations

Dec 24, 2019·Cellular & Molecular Biology Letters·Rafal Bartoszewski, Aleksander F Sikorski
Aug 18, 2020·Cancer Management and Research·Xiaobo Xu, Shusen Zheng
Jun 18, 2020·OncoTargets and Therapy·Hai-Feng MaDa-Hai Zhang
Nov 16, 2019·Cellular & Molecular Biology Letters·Meihua WangYun Cao
Oct 28, 2020·Molecular and Cellular Biochemistry·Libing WangXiaogang Liu
Nov 6, 2020·Cancer Management and Research·Shiqiang FangXiaozhen Hu
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Yuanshen MaoZhong Wang
Aug 3, 2021·Computational and Mathematical Methods in Medicine·Xuhui WuJichun Liu
Oct 17, 2021·Journal of Clinical Laboratory Analysis·Rubing LiFeng Wang

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Methods Mentioned

BETA
transfections
PCR
transfection

Software Mentioned

TargetScan
ImageJ
SPSS

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