PMID: 2108728Apr 9, 1990Paper

Inhibition of mucin secretion in a colonic adenocarcinoma cell line by DIDS and potassium channel blockers

Biochimica Et Biophysica Acta
M A MarconG Forstner

Abstract

The factors which influence the exocytosis of mucins are not well characterized. Since the physical properties of mucins may be affected significantly by the co-secretion of electrolytes and water, we studied the relationship between ion movement and mucin secretion in T84 cells, a human colonic adenocarcinoma cell line which has been well characterized with respect to apical chloride secretion. Secretion of mucin was assessed by immunoassay of mucin appearing in the medium within 30 min of stimulation. Cells were grown on plastic in DMEM/Ham's F12 medium and experiments were carried out at 70% confluence. Mucin secretion was stimulated by the calcium ionophore A23187, or A23187 plus vasoactive intestinal polypeptide. Stimulated mucin secretion was not affected by loop diuretics (furosemide (1 x 10(-3) M) or bumetanide (1 x 10(-4) M)), with or without the addition of ouabain (5 x 10(-5) M) and amiloride (1 x 10(-5) M), making it unlikely that transcellular chloride movements in necessary for mucin secretion. However, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS; (1 x 10(-5) and 5 x 10(-5) M) and three potassium channel blockers BaCl2 (1 x 10(-3) and 5 x 10(-3) M), tetraethylammonium chloride (1 x 10(-2) M) and quinine...Continue Reading

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Citations

Mar 1, 1995·In Vitro Cellular & Developmental Biology. Animal·E HollandeJ Bara
Dec 5, 2002·European Journal of Pharmacology·Gisela KuhnErwin Scharrer
May 1, 1994·The Journal of Clinical Investigation·M E HuflejtT E Machen
Oct 31, 2000·Journal of Cellular Physiology·N A Bradbury
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Vanessa Liévin-Le Moal, Alain L Servin
Nov 8, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jason KovacStephen Vanner
Jan 25, 2000·American Journal of Physiology. Cell Physiology·D R Halm, S T Halm
Sep 14, 1999·The American Journal of Physiology·D R Halm, S T Halm
Feb 9, 1995·Biochimica Et Biophysica Acta·L Liu, A Chander

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