Inhibition of murine T cell-mediated cytolysis and T cell proliferation by a rat monoclonal antibody immunoprecipitating two lymphoid cell surface polypeptides of 94 000 and 180 000 molecular weight

European Journal of Immunology
M PierresP Golstein

Abstract

The monoclonal antibody methodology was use to identify membrane structures involved in T cell functions. To optimize chances to produce and detect relevant antibodies, a xenogeneic sensitization protocol was utilized and hybridoma supernatants were screened, on functional rather than structural grounds, for their ability to inhibit a given function. The test function was T cell-mediated cytolysis. Mouse cytolytic anti-allogeneic cell populations were used to sensitize a rat, the spleen cells of which were fused to produce hybridomas; the supernatants of the latter were screened for their ability to inhibit mouse T cell-mediated cytolysis in vitro. Several inhibitory antibodies were obtained, one of which, H35-89.9 monoclonal antibody, was studied in more detail. It inhibited specific and concanavalin A (Con A)-mediated cytolysis by T cells, by acting on the effector cells. It reversibly inhibited soluble antigen-, alloantigen and Con A-induced T cell proliferation (but not LPS-induced B cell proliferation), after the production of interleukin 2, by acting on the responder cells. It also had a desagglutinating effect on Con A and LPS blasts and on EL4 cells. In immunoprecipitated from thymocyte membrane preparations two structu...Continue Reading

References

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M PierresR N Germain
Apr 1, 1979·European Journal of Immunology·T SpringerC Milstein
Jul 1, 1979·European Journal of Immunology·K F Lindahl, H Lemke
Feb 1, 1975·The Journal of Cell Biology·A L Hubbard, Z A Cohn
Jan 1, 1977·Contemporary Topics in Immunobiology·P Golstein, E T Smith
Jan 1, 1979·Immunological Reviews·J A Ledbetter, L A Herzenberg
Jul 19, 1979·Nature·E L Larsson, A Coutinho
Oct 1, 1978·European Journal of Immunology·D W Mason, G G Gallico
Feb 1, 1973·The Journal of Experimental Medicine·C Neauport-SautesF M Kourilsky
Mar 1, 1974·Journal of Immunological Methods·R M ThornL A Manson
Feb 1, 1972·Cellular Immunology·R E ClickB J Alter
Jan 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·P Coffino, M D Scharff
Oct 1, 1980·The Journal of Experimental Medicine·E A LernerD B Murphy
Oct 1, 1980·European Journal of Immunology·E L ReinherzS F Schlossman
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E NakayamaL J Old
Sep 1, 1980·The Journal of Experimental Medicine·N HollanderI L Weissman
Feb 1, 1981·Journal of Immunogenetics·M PierresM Dosseto
May 1, 1980·The Journal of Experimental Medicine·M B OmaryM P Scheid

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Citations

Jan 1, 1993·Stem Cells·S de Kossodo, G E Grau
Jan 1, 1982·Immunogenetics·M SuzanB Rubin
Jul 1, 1991·Journal of Clinical Immunology·L M VossP J Leibson
Sep 1, 1991·The Histochemical Journal·J P De JongR E Ploemacher
Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·M J PalissonP Bischoff
Jan 1, 1982·Springer Seminars in Immunopathology·J W Goding
Sep 14, 1994·Journal of Immunological Methods·P J LeenenW van Ewijk
Nov 1, 1986·Molecular Immunology·R BurgerH Schaefer
May 1, 1993·Molecular Immunology·C LeclercV Deubel
Jun 1, 1983·Journal of Neuroimmunology·M R HirschC Goridis
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·C G GahmbergM Patarroyo
Jul 30, 2002·European Journal of Haematology·Pierre F Piguet, Christian Vesin
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P MachyL D Leserman
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G Marchal, G Milon
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L RéniaD Mazier
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·P Velupillai, D A Harn
Oct 1, 1982·The Journal of Experimental Medicine·D I BellerR D Schreiber
Mar 1, 1990·The Journal of Experimental Medicine·C R MackayL Dudler
Mar 1, 1994·The Journal of Experimental Medicine·A O HueberH T He
Mar 1, 1995·The Journal of Experimental Medicine·A H Shankar, R G Titus
Apr 1, 1996·The Journal of Experimental Medicine·R SchmitsD D Hickstein

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