PMID: 7537591Feb 1, 1995Paper

Inhibition of muscarinic receptor-induced inositol phospholipid hydrolysis by caffeine, beta-adrenoceptors and protein kinase C in intestinal smooth muscle

British Journal of Pharmacology
S A Prestwich, T B Bolton

Abstract

1. The effects of caffeine, isoprenaline, dibutyryl cyclic AMP, isobutylmethylxanthine (IBMX), 12-O-tetradecanoylphorbol-13-acetate (TPA) or 1-oleoyl-2-acetylglycerol (OAG), (protein kinase C (PKC) activators), 2-methoxy verapamil (D600), thapsigargin and ryanodine on muscarinic acetylcholine receptor (AChR)-stimulated inositol phospholipid hydrolysis were studied in smooth muscle fragments from the longitudinal layer of the small intestine of the guinea-pig. 2. Incubation of the fragments with the muscarinic agonist, carbachol (CCh) (100 microM) resulted in rapid increases in the levels of all the inositol phosphate isomers with maximal increases in the [3H]-inositol (1,4,5) trisphosphate ([3H]-Ins(1,4,5)P3) isomer occurring 10 s following incubation. 3. The beta-adrenoceptor agonist, isoprenaline (10 microM) and dibutyryl cyclic AMP (10 microM), a membrane permeant analogue of cyclic AMP both reduced the CCh stimulation, but not the basal levels of [3H]-inositol phosphates. This inhibition by dibutyryl cyclic AMP was enhanced in the presence of the phosphodiesterase inhibitor, IBMX. CCh inhibited the isoprenaline-induced increases in the levels of cyclic AMP and this was via a pertussi toxin (PTX)-sensitive G-protein mechanis...Continue Reading

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Citations

Feb 12, 1998·British Journal of Pharmacology·A V Zholos, T B Bolton
Aug 1, 1996·The Journal of Pharmacy and Pharmacology·M ElorriagaD Ivorra
Oct 8, 2011·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Yuhsuke ShigemasaHikaru Suzuki
Jan 1, 1997·Life Sciences·F J EhlertG W Sawyer
Dec 23, 2004·The Journal of Physiology·F R Edwards, G D S Hirst
May 29, 2002·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·C Kemal Buharalioğlu, Fatma Akar
Jul 1, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Takayuki MatsumotoKatsuo Kamata
Sep 9, 2004·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Eri NakamuraHikaru Suzuki
Jan 21, 2010·Physiological Reviews·Susan Wray, Theodor Burdyga
May 2, 2002·The Journal of Biological Chemistry·Irina StartchikMichel F Rossier
Aug 15, 2001·American Journal of Physiology. Cell Physiology·C W McLaughlinM M Civan
Jan 19, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Eri NakamuraHikaru Suzuki
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·S ChakderS Rattan
Jan 14, 1999·The American Journal of Physiology·P E SquiresA M Buchan

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