Inhibition of Na Transport by prostacyclin (PGI2) in rabbit cortical collecting tubule

Prostaglandins
Y Iino, B M Brenner

Abstract

The effects of prostacyclin (PGI2) on transepithelial potential difference (PD) and sodium transport were examined in rabbit cortical collecting tubules (CCT) perfused in vitro. Addition of PGI2 (10-6M) to the bathing medium, which was bubbled with 95% O2 - 5% CO2, caused a reversible decrease in PD averaging 49 +/- 9.4(SE)%. Maximal effect was evident between 5-10 min. after addition of PGI2 and PD returned spontaneously towards control values within 30 min., corresponding to the rapid degradation of PGI2. In a more alkaline bathing solution achieved by bubbling with 100% O2, in which the degradation of PGI2 is known to be delayed markedly, the decrease in PD by PGI2 was continuous and dose-dependent, with half-maximal and maximal effects achieved at 10-7M and 10-5M, respectively. Neither 10-8M PGI2 nor vehicle alone exerted significant effects on PD. 6-keto-PGF1 alpha (10-5M), believed to be the major metabolite of PGI2, had no effect on PD. Lumen-to-bath flux of Na decreased with PGI2 from 9.0 to 5.6 pEq/cm/sec (n=4, p less than 0.005), although bath-to-lumen flux did not change significantly. In summary, PGI2 caused a dose-dependent decrease in PD of rabbit CCT and inhibited Na absorption in this segment in vitro. These res...Continue Reading

Citations

Jun 1, 1991·Pflügers Archiv : European journal of physiology·M SteidlF Lang
Dec 16, 1998·Kidney International·M KömhoffR M Nüsing
Oct 10, 2002·Kidney International·Helga VitzthumArmin Kurtz
Jan 1, 1985·Medicinal Research Reviews·R C NickolsonH Vorbrüggen
Apr 1, 1983·Klinische Wochenschrift·R DüsingH J Kramer
Feb 1, 1982·The American Journal of Medicine·D J LevensonB M Brenner

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