PMID: 9653774Jul 8, 1998Paper

Inhibition of Na+,K(+)-ATPase by platelet-activating factor in dog submandibular glands

Prostaglandins & Other Lipid Mediators
K ItadaniT Dohi

Abstract

Physiological stimulation of dog submandibular gland has been shown to generate platelet-activating factor (PAF). However, PAF is not released from cells in the tissue. To assess its intracellular activity, the effect of PAF on Na+,K(+)-ATPase was examined in dog submandibular gland cells. PAF inhibited Na+,K(+)-ATPase in membrane preparations, and the inhibitory effect was dependent on the protein concentration in the enzyme preparation. The inhibitory effect of a low concentration of PAF was antagonized by a PAF-receptor antagonist, BN 50,739, but at high concentrations, PAF was not antagonized. Kinetic analysis of PAF inhibition of Na+,K(+)-ATPase suggests that the inhibition of Na+,K(+)-ATPase by PAF is not due to competition by PAF at K(+)- or Na(+)-binding sites on the enzyme, but by complex inhibitory mechanisms. These results suggest that PAF may interact with specific and nonspecific site of action resulting in the inhibition of Na+,K(+)-ATPase. Ouabain increased mucin release from dog submandibular gland cells. Because Na+,K(+)-ATPase and ion exchange pathways are important in the secretory responses of acinar cells, PAF may regulate intracellularly the secretory function of acinar cells by modulating Na+,K(+)-ATPase ...Continue Reading

References

Oct 29, 1987·Biochemical and Biophysical Research Communications·P PatrignaniP Musiani
Oct 1, 1967·Acta Physiologica Scandinavica·O H Petersen, J H Poulsen
Dec 1, 1984·The Biochemical Journal·M A McPherson, R L Dormer

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Citations

Jan 17, 2003·American Journal of Physiology. Renal Physiology·Rajash K HandaShelly E Handa

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