PMID: 8607259Feb 15, 1996Paper

Inhibition of Na+K+ATPase activity in membranes of Sindbis virus-infected chick cells

Virology
E T UlugH R Bose

Abstract

Alterations in intracellular concentrations of Na+ and K+ in Sindbis virus-infected cells result largely from inhibition of ouabain-sensitive Na+K+ATPase (Na+ pump) activity. Here we report that membrane preparations derived from Sindbis virus-infected chick cells exhibit reduced Na+K+ATPase activity, indicating that limitation of cellular factors is not responsible for inhibition of ion transport. In vitro phosphorylation of the Na+K+ATPase by [32P]orthophosphate or [gamma 32P]ATP is unaltered in membranes of Sindbis virus-infected cells, indicating that a loss of specific enzymatic functions unrelated to formation of Na+ pump phosphoenzyme intermediates occurs during the course of viral infection. However, phosphoenzyme intermediates of the Na+K+ATPase prepared from membranes of Sindbis virus-infected cells are inherently less stable than those prepared with membranes of uninfected cells. The instability of these intermediates in vitro is correlated with an altered capacity of the Na+ pump to transport monovalent cations into virus-infected cells. 22Na+ transport studies reveal enhanced ouabain-sensitive Na+ uptake by Sindbis virus-infected cells, suggesting that the Na+ pump may catalyze enhanced Na+-Na+ exchange in the infe...Continue Reading

Citations

Oct 20, 2007·Virology Journal·Joshua M Costin
May 29, 2020·BioMed Research International·Kauê F C Souza-SouzaPatrícia Burth
Jul 26, 2018·International Journal of Molecular Sciences·Luciano Amarelle, Emilia Lecuona
Nov 14, 1998·The American Journal of Physiology·L M MaglovaJ M Russell
Sep 5, 2021·Naunyn-Schmiedeberg's Archives of Pharmacology·Hope OnohueanGaber El-Saber Batiha

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