Inhibition of neuronal nitric oxide synthase by 6-nitrocatecholamines, putative reaction products of nitric oxide with catecholamines under oxidative stress conditions

The Biochemical Journal
Anna PalumboMarco d'Ischia

Abstract

6-Nitrodopamine and 6-nitronoradrenaline (6-nitronorepinephrine), putative products of the nitric oxide (NO)-dependent nitration of dopamine and noradrenaline, are reported to be reversible, competitive inhibitors of neuronal nitric oxide synthase (nNOS) with K(i) values of 45 and 52 microM respectively. The nitrocatecholamines inhibited H(2)O(2) production in the absence of L-arginine and tetrahydrobiopterin (BH(4)) (the IC(50) values for 6-nitrodopamine and 6-nitronoradrenaline were 85 and 55 microM respectively) but without affecting cytochrome c reduction. The apparent K(i) values for nitrocatecholamine inhibition of enzyme activation by BH(4) were 18 microM for 6-nitrodopamine and 40 microM for 6-nitronoradrenaline. Both nitrocatecholamines antagonized the dimerization of nNOS induced by BH(4) and by L-arginine, the effect being reversed by BH(4) (more than 10 microM) and L-arginine (e.g. 100 microM). Overall, these results suggest that nitrocatecholamines interfere with nNOS activity by binding to the enzyme in the proximity of the substrate and BH(4)-binding sites near the haem group.

References

Nov 13, 1995·Biochemical and Biophysical Research Communications·H MacarthurT C Westfall
Jun 1, 1994·Archives of Biochemistry and Biophysics·D J Wolff, B J Gribin
Jul 1, 1995·Bioorganic & Medicinal Chemistry·M d'Ischia, C Costantini
Jun 7, 1996·The Journal of Biological Chemistry·F ShintaniT Nakaki
Jan 1, 1996·Advances in Neuroimmunology·Y Liu
Mar 1, 1997·Trends in Neurosciences·C Iadecola
Jan 20, 1998·Trends in Biochemical Sciences·B Mayer, B Hemmens
Aug 24, 1999·The Journal of Biological Chemistry·A ReifH H Schmidt

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