Inhibition of neuronal nitric oxide synthase by 7-nitroindazole attenuates acute lung injury in an ovine model

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Perenlei EnkhbaatarD L Traber

Abstract

Nitric oxide (NO) has been shown to play a major role in acute lung injury (ALI) after smoke inhalation. In the present study, we developed an ovine sepsis model, created by exposing sheep to smoke inhalation followed by instillation of bacteria into the airway, that mimics human sepsis and pneumonia. We hypothesized that the inhibition of neuronal NO synthase (nNOS) might be beneficial in treating ALI associated with this model. Female sheep (n = 26) were surgically prepared for the study and given a tracheostomy. This was followed by insufflation of 48 breaths of cotton smoke (40 degrees C) into the airway of each animal and subsequent instillation of live Pseudomonas aeruginosa [5 x 10(11) colony forming units (CFU)] into each sheep's lung. All sheep were mechanically ventilated using 100% O2. Continuous infusion of 7-nitroindazole (7-NI), an nNOS inhibitor, NG-monomethyl-l-arginine (l-NMMA), a nonspecific NOS inhibitor, or aminoguanidine (AG), an inducible NOS inhibitor, was started 1 h after insult. The administration of 7-NI improved pulmonary gas exchange (PaO2/FiO2; where PaO2 is arterial PO2 and FiO2 is fractional inspired oxygen concentration) and pulmonary shunt fraction and attenuated the increase in lung wet-to-dry...Continue Reading

References

Aug 11, 1991·Journal of Applied Physiology·S AbdiD L Traber
Nov 11, 1991·The Journal of Trauma·G B HubbardB A Pruitt
Nov 1, 1990·Journal of Applied Physiology·J C StothertD L Traber
Nov 1, 1990·The Journal of Burn Care & Rehabilitation·S AbdiD L Traber
May 1, 1990·Journal of Applied Physiology·R E BarrowD N Herndon
Sep 1, 1989·Journal of Applied Physiology·C A HalesJ Burke
May 1, 1988·The American Review of Respiratory Disease·A ReyesP D Wagner
Jan 1, 1987·Annals of Surgery·K Z ShiraniA D Mason
May 1, 1994·Journal of Vascular Research·W C Sessa
Jul 1, 1994·British Journal of Pharmacology·D Minc-GolombJ P Schwartz
Jan 1, 1994·Annual Review of Biochemistry·D S Bredt, S H Snyder
Aug 1, 1994·American Journal of Respiratory and Critical Care Medicine·H IschiropoulosS R Thom
Feb 1, 1996·American Journal of Respiratory and Critical Care Medicine·S L SoodD Easa
Jul 1, 1996·American Journal of Respiratory and Critical Care Medicine·A Fischer, B Hoffmann
Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·S R HopkinsP D Wagner
Oct 6, 1997·American Journal of Respiratory and Critical Care Medicine·S R FischerD L Traber
Nov 14, 1997·The American Journal of Physiology·G T De SanctisJ M Drazen
Apr 16, 1998·Journal of Applied Physiology·H SakuraiD L Traber
Dec 16, 1998·American Journal of Respiratory and Critical Care Medicine·A S KristofS N Hussain
Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·M FeletouE Canet
Sep 28, 2002·Critical Care Medicine·Kazunori MurakamiDaniel L Traber

❮ Previous
Next ❯

Citations

Aug 9, 2007·Intensive Care Medicine·Xizhong CuiZenaide M N Quezado
Dec 4, 2008·Critical Care Medicine·Perenlei EnkhbaatarDaniel L Traber
Dec 4, 2008·Critical Care Medicine·Matthias LangePerenlei Enkhbaatar
Jun 12, 2009·Der Anaesthesist·M O MaybauerD M Maybauer
Jan 6, 2016·International Journal of Molecular Sciences·Ashley N GuilloryCeleste C Finnerty
Jun 19, 2013·Burns : Journal of the International Society for Burn Injuries·Matthias LangePerenlei Enkhbaatar
Jan 5, 2011·Burns : Journal of the International Society for Burn Injuries·Naoki MoritaDaniel L Traber
Oct 2, 2007·Burns : Journal of the International Society for Burn Injuries·Arti PariharSatyanarayan Bhat
Nov 19, 2003·Biochemical and Biophysical Research Communications·Martin WestphalDaniel Lee Traber
Apr 15, 2004·Biochemical and Biophysical Research Communications·Martin WestphalDaniel Lee Traber
Dec 28, 2010·Biochemical and Biophysical Research Communications·Matthias LangePerenlei Enkhbaatar
May 12, 2006·Redox Report : Communications in Free Radical Research·Naoki MoritaDaniel L Traber
Jul 27, 2010·Biochemical and Biophysical Research Communications·Matthias LangePerenlei Enkhbaatar
Jun 10, 2011·European Journal of Pharmacology·Danielle DumaJamil Assreuy
Jan 17, 2017·Pituitary·Camila Henriques CoelhoMaria José Alves da Rocha
Mar 16, 2006·The Journal of Pathology·N HopkinsP McLoughlin
Sep 26, 2006·Journal of Burn Care & Research : Official Publication of the American Burn Association·Deborah L Carlson, Jureta W Horton
Jun 10, 2004·Critical Care Medicine·Martin WestphalDaniel L Traber
May 18, 2011·Shock·Charles E Wade, René M Sauer
Nov 15, 2008·Shock·Perenlei EnkhbaatarDaniel Traber
Dec 9, 2008·Journal of Burn Care & Research : Official Publication of the American Burn Association·Daniel L TraberDavid N Herndon
Apr 8, 2009·Journal of Burn Care & Research : Official Publication of the American Burn Association·Robert A CoxHal K Hawkins
Jul 23, 2005·Anesthesia and Analgesia·Balázs HauserBalasubramanian Venkatesh
Dec 24, 2005·Shock·Daniel L TraberLillian D Traber
Dec 30, 2008·Critical Care Medicine·John A Dawson
May 21, 2004·Clinical Science·Perenlei Enkhbaatar, Daniel L Traber
Jul 21, 2009·Biochemical Society Transactions·Christian HesslingerClemens Braun
May 9, 2006·Shock·Perenlei EnkhbaatarDaniel L Traber
May 6, 2021·Burns : Journal of the International Society for Burn Injuries·Suzan AlharbiPerenlei Enkhbaatar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.