Abstract
To explore the role of NF-κB activation in the development of insulin resistance and investigate whether or not that the inhibition of NF-κB activation by PDTC will improve the insulin resistance of L6 cells exposed to H2O2. L6 cells were treated with H2O2, PDTC or both H2O2 and PDTC for 4 hours. The uptake of glucose with stimulation of insulin, the expression of P38-MAPK, p- P38-MAPK, NF-κBp65, p- NF-κBp65, IRS-1, IRS-2, p-IRS-2, PI3K, IκBα, p- IκBα, caspase-8 and GLUT4, the production of ROS, TNF-α, IL-6 and IL-1β as well as the apoptosis rate of L6 cells were determined and compared in L6 treated with H2O2 alone or both H2O2 and PDTC. Compared with the L6 cells treated with H2O2 alone, the L6 cells treated with both H2O2 and PDTC showed (1) significantly lower production of ROS, TNF-α, IL-6 and IL-1β; (2) significantly decreased expression of P38-MAPK, p- P38-MAPK and NF-κBp65, p- NF-κBp65, p- IκBα and caspase-8; (3) significantly lower rate of apoptosis; (4) significantly higher expression of IRS-2, p-IRS-2 (Tyr 612), PI3K and GLUT4; (5) significantly higher uptake of glucose with stimulation of insulin; (6) significantly increased expression of Bcl2 and decreased ratio of Bax to Bcl2. Based on the findings of the present ...Continue Reading
References
Mar 1, 1992·Diabetes Care·R A DeFronzoE Ferrannini
Jan 1, 1996·Free Radical Biology & Medicine·S LenzenM Tiedge
Aug 15, 1997·The Journal of Biological Chemistry·B S Berlett, E R Stadtman
Nov 23, 2000·Nature·T Finkel, N J Holbrook
May 4, 2001·Diabetes·S YonemitsuK Nakao
Jun 28, 2002·Free Radical Biology & Medicine·Rajindar S Sohal
Dec 28, 2002·Diabetes·Joseph L EvansGerold M Grodsky
Apr 28, 2004·Diabetes Care·Sarah WildHilary King
May 6, 2004·Diabetologia·T OgiharaT Fujita
Jul 11, 2006·The Journal of Clinical Investigation·Steven E ShoelsonAllison B Goldfine
Dec 15, 2006·Nature·Steven E KahnKristina M Utzschneider
Oct 27, 2009·Trends in Endocrinology and Metabolism : TEM·Dorit Samocha-BonetLesley V Campbell
Apr 22, 2010·Basic & Clinical Pharmacology & Toxicology·Monika ChabicovskyOtto Doblhoff-Dier
Jun 8, 2011·PloS One·Nasser SamadiDavid N Brindley
Sep 6, 2011·Trends in Molecular Medicine·Miriam CnopLicio A Velloso
Nov 8, 2011·Free Radical Biology & Medicine·Jennalynn StyskalAdam B Salmon
Sep 7, 2012·Diabetes, Obesity & Metabolism·G Drews, M Düfer
Nov 12, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Samantha E Stokes, Louise M Winn
Citations
Sep 11, 2020·Journal of Food Biochemistry·Tingxu YanZhenzhong Wang
Feb 7, 2019·Frontiers in Physiology·Rodrigo Vanerson Passos NevesMilton Rocha Moraes
Oct 24, 2018·Life Sciences·Milton Rocha MoraesNiels Olsen Saraiva Câmara