Inhibition of NF-κB Activity Enhances Sensitivity to Anticancer Drugs in Cholangiocarcinoma Cells.

Oncology Research
Wunchana SeubwaiSopit Wongkham

Abstract

Cholangiocarcinoma (CCA) is a dismal cancer. At present, there is no effective chemotherapeutic regimen for CCA. This may be due to the marked resistance of CCA to chemotherapy drugs, for which a mechanism remains unknown. Nuclear factor-κB (NF-κB) is constitutively activated in a variety of cancer cells, including CCA. It has been shown to play roles in growth, metastasis, and chemoresistance of cancer. In the present study, we examined whether NF-κB is involved in the chemoresistance of CCA and whether dehydroxymethylepoxyquinomicin (DHMEQ), an effective NF-κB inhibitor, can overcome the drug resistance of CCA. Two CCA cell lines, KKU-M213 and KKU-M214, were treated with DHMEQ and/or chemotherapeutic drugs. Cell viability, apoptosis, and the expressions of the ATP-binding cassette (ABC) transporters were compared. The combination of chemotherapy drugs, 5-fluorouracil, cisplatin, and doxorubicin, with DHMEQ significantly enhanced the cytotoxicity of all chemotherapeutic drugs compared to DHMEQ or drug alone. Furthermore, the mRNA level of ABCB1, a multidrug-resistant protein, was significantly decreased in the 5-fluorouracil combined with DHMEQ-treated cells. These findings suggest that the inhibition of NF-κB by DHMEQ enhance...Continue Reading

References

Jun 8, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J E RosM Müller
Aug 8, 2001·Surgery·C B WeldonB S Beckman
Mar 31, 2004·Journal of the American College of Surgeons·Bridget N FahyRichard J Bold
Jun 11, 2005·World Journal of Gastroenterology : WJG·Banchob SripaMasanao Miwa
Feb 3, 2007·Cancer Science·Jun-Ichiro InoueKentaro Semba
Jul 16, 2008·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Sara Vander BorghtTania A Roskams
Mar 12, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kei SakamotoMasao Omata
May 14, 2009·Journal of Radiation Research·Rakhesh MadhusoodhananNatarajan Aravindan
Apr 24, 2010·Cancer Science·Wunchana SeubwaiSopit Wongkham
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Wenshu ChenYong Lin
Jan 29, 2011·Current Gastroenterology Reports·Halim Charbel, Firas H Al-Kawas
Jul 26, 2011·Archivum Immunologiae Et Therapiae Experimentalis·Sanjay BatraMalaya K Sahoo
Aug 23, 2011·The International Journal of Biochemistry & Cell Biology·Christudas MoraisHelen Healy
Nov 17, 2011·World Journal of Urology·Garima JainRalf B Marienfeld
Apr 13, 2012·Expert Opinion on Investigational Drugs·Marco Falasca, Kenneth J Linton
Jun 15, 2012·Biochemical and Biophysical Research Communications·Yuji SakumaYohei Miyagi
Jun 30, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Jingwei ZhangGuangji Wang
Aug 17, 2012·Gut·Shahid A KhanUNKNOWN British Society of Gastroenterology
Nov 14, 2012·The Journal of Pharmacy and Pharmacology·Can HuangYong Su
Aug 7, 2013·World Journal of Gastrointestinal Oncology·Natalia Ramírez-MerinoHernán Cortés-Funes
Jan 11, 2014·Journal of Hepato-biliary-pancreatic Sciences·Paiboon SithithawornChawalit Pairojkul

❮ Previous
Next ❯

Citations

Jun 2, 2017·Biological & Pharmaceutical Bulletin·Nattapong PuthdeeSopit Wongkham
Jun 11, 2017·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Jose J G MarinRocio I R Macias
Nov 1, 2018·The Journal of Biological Chemistry·Takehiko TezukaHideto Watanabe
Sep 25, 2017·Oncotarget·Hua-Chuan Zheng
Apr 5, 2018·Frontiers in Immunology·Jin'en WuYadong Zheng
Dec 9, 2020·Bioorganic & Medicinal Chemistry·Teerapich KasemsukRungnapha Saeeng
Feb 6, 2020·International Journal of Molecular Sciences·Kornkamon LertsuwanJomnarong Lertsuwan

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Assay
PCR

Software Mentioned

IN Cell Analyzer Workstation
Primer
- BLAST
IN Cell Analyzer
IN Cell Analyzer 2000

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis