Inhibition of NF-kappa B binding to DNA by chromium, cadmium, mercury, zinc, and arsenite in vitro: evidence of a thiol mechanism

Archives of Biochemistry and Biophysics
J A ShumillaA Barchowsky

Abstract

NF-kappa B binding to DNA in the presence of thiol-reactive metals has been explored in vitro. Gel mobility shift assays using total nuclear extracts isolated from tumor necrosis factor alpha-treated A549 cells demonstrated dose-dependent inhibition of NF-kappa B binding by chromium, cadmium, mercury, zinc, and arsenite. Maximum inhibition of binding occurred when these metals were preincubated with the nuclear proteins prior to addition of radiolabeled oligonucleotide. The potency of mercury, cadmium, and zinc for inhibiting binding closely correlated to the affinity of these metals for protein thiols. Further addition of dithiothreitol competitively blocked the effects of all of the metals, except chromium(III), on NF-kappa B binding. This study demonstrates mechanisms for metals to inhibit NF-kappa B-DNA binding through interactions with critical protein sulfhydryls.

References

Oct 15, 1979·Archives of Biochemistry and Biophysics·A G Splittgerber, A L Tappel
Jan 1, 1992·Annual Review of Biochemistry·C O Pabo, R T Sauer
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M B Toledano, W J Leonard
Dec 1, 1986·Archives of Biochemistry and Biophysics·A WedrychowskiL S Hnilica
Jan 1, 1980·Environmental Mutagenesis·T G RossmanW Troll
Nov 15, 1995·Genes & Development·I M VermaS Miyamoto
Feb 1, 1995·Free Radical Biology & Medicine·S J Stohs, D Bagchi
Feb 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S MihmW Dröge
Jan 26, 1995·Nature·C W MüllerS C Harrison
Feb 24, 1995·Cell·D Thanos, T Maniatis
Mar 1, 1996·Nucleic Acids Research·J S Hanas, C G Gunn
May 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·A Fluharty, D R Sanadi

❮ Previous
Next ❯

Citations

Oct 31, 2006·Oncogene·T D Gilmore, M Herscovitch
Mar 3, 1999·The Biochemical Journal·M Djavaheri-MergnyL Dubertret
Aug 9, 2002·Journal of Toxicology and Environmental Health. Part a·Song-Lih HuangChang-Chuan Chan
Jun 30, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chad M ThompsonMark A Harris
Jul 14, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michael F HughesDavid J Thomas
Sep 28, 2006·Annual Review of Pharmacology and Toxicology·Yoshito Kumagai, Daigo Sumi
Sep 14, 2011·Journal of Toxicology·Eman M Alissa, Gordon A Ferns
Nov 12, 2002·Environmental Health Perspectives·Fei Chen, Xianglin Shi
Dec 4, 2003·Journal of Occupational Health·Yong-Dae KimHeon Kim
Mar 26, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·P L AbreuA M Urbano
May 13, 2006·Marine Environmental Research·Jonathan A Roling, William S Baldwin
Apr 26, 2011·Environmental Pollution·Sara C NovaisMónica J B Amorim
Sep 3, 2014·Redox Biology·Miriam M Cortese-KrottChristoph V Suschek
Oct 15, 2013·International Journal of Environmental Health Research·Anju RaniManu Pant
Jun 20, 2006·The Journal of Nutritional Biochemistry·Jyrki K VirtanenTomi-Pekka Tuomainen
Mar 3, 2010·Toxicology and Applied Pharmacology·Daigo SumiYoshito Kumagai
Dec 29, 2000·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·A V Kudrin
Jul 2, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hong MeiYingying Le
Nov 13, 2003·Journal of Biomedical Materials Research. Part a·J B LewisP E Lockwood
Jun 16, 2006·Journal of Cellular Physiology·Kimberley A O'HaraAaron Barchowsky
Jun 29, 2005·Journal of Biomedical Materials Research. Part a·J B LewisW-Y Tseng
Apr 12, 2011·Photochemistry and Photobiology·Yu WangHidefumi Akiyama
Feb 16, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jianxun Xie, Zahir A Shaikh
Dec 22, 2010·Medical Microbiology and Immunology·Nadine WiesenerAndreas Henke
Aug 9, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jessica Napolitano CoxDaren L Knoell
Jul 17, 2020·Carcinogenesis·Yusha Zhu, Max Costa
May 26, 2018·Journal of Fungi·Alexander Batista-DuharteIracilda Zeppone Carlos
Apr 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Weidong WuJames M Samet
May 10, 2000·The Journal of Nutrition·E Mocchegiani, M Muzzioli
Mar 24, 2021·Current Environmental Health Reports·Denisse DiazIvan A Arenas
Apr 3, 2021·Seminars in Cancer Biology·Uttara SaranChendil Damodaran

❮ Previous
Next ❯

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