PMID: 3316462Dec 1, 1987Paper

Inhibition of NK and ADCC activity by antibodies against purified cytoplasmic granules from rat LGL tumors

Journal of Leukocyte Biology
C W ReynoldsP A Henkart

Abstract

Highly purified preparations of cytoplasmic granules from transplantable rat large granular lymphocyte (LGL) tumor lines (rat natural killer (RNK) tumors) were used to immunize rabbits. Antibodies from these animals gave two precipitin lines with granule extracts in Ouchterlony experiments. They reacted with at least four different bands on nitrocellulose blots of SDS gels of LGL granule proteins. By immunofluorescence, specifically adsorbed antigranule antibodies did not recognize LGL or T cell surface antigens but reacted with the cytoplasmic granules in permeabilized RNK tumor cells as well as with normal rat LGL. These same antisera showed little or no reactivity with a panel of other cells, including peripheral blood T cells, thymocytes, macrophages, and EL-4 tumor cells. F(ab')2 preparations of these antigranule antibodies completely blocked granule-mediated lysis of both SRBC and nucleated targets, while control F(ab')2 preparations from rabbits immunized with EL-4 granules or TNP-KLH showed no significant inhibition of this cytolytic activity at the same antibody concentration. Anti-granule F(ab')2 preparations specifically inhibited (greater than 75%) rat natural killer (NK) and antibody-dependent cellular cytotoxicity...Continue Reading

Citations

Jul 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K VanderkerkenE Wisse
Nov 1, 1991·Molecular Immunology·A E KutaP A Henkart
May 1, 1988·Immunology Today·J D Young, C C Liu

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