Inhibition of NLRP3 Inflammasome Ameliorates Cerebral Ischemia-Reperfusion Injury in Diabetic Mice.

Neural Plasticity
Pu HongHong-Fei Zhang

Abstract

Sustained activation of NLRP3 inflammasome is closely related to diabetes and stroke. However, it is unknown whether NLRP3 inflammasome plays an essential role in stroke in diabetes. We aim to investigate the effect and the potential mechanism of NLRP3 inflammasome in diabetic mice with cerebral ischemia-reperfusion injury. A type 2 diabetic mouse model was induced by a high-fat diet and streptozotocin (STZ). Diabetic mice received MCC950 (the specific molecule NLRP3 inhibitor) or vehicle 60 minutes before the middle cerebral artery occlusion (MCAO) and reperfusion. MCC950 reduced the neurological deficit score of 24 h after cerebral ischemia reperfusion and improved the 28-day survival rate of cerebral ischemia-reperfusion injury in diabetic mice. Furthermore, we found that the mRNA transcription levels of NLRP3, IL-1β, and caspase-1 in the core ischemic area were remarkably amplified in diabetic mice with cerebral ischemia-reperfusion injury, whereas this phenomenon was obviously attenuated by MCC950 pretreatment. In conclusion, the NLRP3 inflammasome was involved in the complex diseases of diabetic stroke. MCC950, the NLRP3 specific inhibitor, ameliorated diabetic mice with cerebral ischemia-reperfusion injury and improved t...Continue Reading

References

Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·E Z LongaR Cummins
Dec 26, 2006·Journal of Neuroimmunology·Qing WangMidori A Yenari
Feb 3, 2009·Stroke; a Journal of Cerebral Circulation·Brad R S BroughtonChristopher G Sobey
Mar 24, 2009·Annual Review of Immunology·Charles A Dinarello
Jan 16, 2010·Science·Kate SchroderJurg Tschopp
Dec 3, 2010·Nature·Rongbin ZhouJürg Tschopp
Aug 25, 2011·Anesthesiology·Julie L W NgAdrian W Gelb
Feb 22, 2012·Lancet Neurology·Merel J A LuitseL Jaap Kappelle
Mar 21, 2013·Neuroscience Bulletin·Shen-Bin LiuYan-Qing Wang
Jan 16, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Fan YangFan Yi
Mar 6, 2015·Nature Medicine·Maayan LevyEran Elinav
Sep 4, 2015·Current Protocols in Pharmacology·Brian L Furman
Feb 24, 2016·Recent Patents on Endocrine, Metabolic & Immune Drug Discovery·Caroline M O VolpeJosé A Nogueira-Machado
Aug 4, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Nikolett LénártÁdám Dénes
Aug 16, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael J PrimianoJ Perry Hall
Dec 14, 2016·British Journal of Anaesthesia·H ZhangX Xiong
Jan 24, 2017·JAMA : the Journal of the American Medical Association·Fiona BraggUNKNOWN China Kadoorie Biobank Collaborative Group
Feb 9, 2017·Journal of Hepatology·Auvro R MridhaGeoffrey C Farrell

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Citations

May 30, 2020·International Journal of Molecular Sciences·Claudia Espinosa-GarciaDonald G Stein
Jun 17, 2020·Experimental and Therapeutic Medicine·Yan-Zi ZhangJi-Hong Chen
Jun 26, 2020·Frontiers in Cellular Neuroscience·Ya-Shuo FengFeng Zhang
Nov 3, 2020·BioFactors·Nuttapong YawootJiraporn Tocharus
Dec 23, 2020·Journal of Cardiovascular Pharmacology·Dong HanYan Gao
Aug 5, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mick FrissenChristian Trautwein
Apr 30, 2021·Neuropsychiatric Disease and Treatment·Junjie LiJianlin Shao
Feb 25, 2021·CNS & Neurological Disorders Drug Targets·Ghaith A Bahadar, Zahoor A Shah

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Methods Mentioned

BETA
PCR

Software Mentioned

ImageJ
Prism5

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