Inhibition of NOX2 signaling limits pain-related behavior and improves motor function in male mice after spinal cord injury: Participation of IL-10/miR-155 pathways

Brain, Behavior, and Immunity
Boris SabirzhanovJunfang Wu

Abstract

NADPH oxidase (NOX2) is an enzyme that induces reactive oxygen species (ROS) and serves as a switch between the pro-inflammatory and neurorestorative microglial/macrophage phenotypes; such changes play an important role in neuropathic pain and motor dysfunction. Increased NOX2 expression after spinal cord injury (SCI) has been reported, and inhibition of NOX2 improves motor function. However, the underlying mechanisms of NOX2 in post-traumatic pain and motor deficit remain unexplored. In the present study, we report that depletion of NOX2 (NOX2-/-) or inhibition of NOX2 using NOX2ds-tat significantly reduced mechanical/thermal cutaneous hypersensitivity and motor dysfunction after moderate contusion SCI at T10 in male mice. Western blot (WB, 3 mm lesion area) and immunohistochemistry (IHC) showed that SCI elevates NOX2 expression predominantly in microglia/macrophages up to 8 weeks post-injury. Deletion of NOX2 significantly reduced CD11b+/CD45hiF4/80+ macrophage infiltration at 24 h post-injury detected by flow cytometry and 8-OHG+ ROS production at 8 weeks post-injury by IHC in both lesion area and lumbar enlargement. NOX2 deficiency also altered microglial/macrophage pro-inflammatory and anti-inflammatory balance towards the...Continue Reading

References

Apr 1, 1996·Physiological Reviews·M E Schwab, D Bartholdi
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Mar 25, 2004·Nature Reviews. Immunology·J David Lambeth
Nov 3, 2004·Archives of Physical Medicine and Rehabilitation·Marca L SipskiAdam Stein
Jan 9, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Dario SiniscalcoVito de Novellis
May 18, 2007·Annual Review of Neuroscience·Mauricio E Vargas, Ben A Barres
Aug 19, 2008·Experimental Neurology·Claire E Hulsebosch
Jan 22, 2009·Brain Research Reviews·Claire E HulseboschSusan M Carlton
May 7, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hai ChenPak H Chan
Jul 25, 2009·Molecular Neurobiology·Marina A Lynch
Oct 30, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kristina A KigerlPhillip G Popovich
May 4, 2010·The Journal of Biological Chemistry·Claire E McCoyLuke A J O'Neill
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Donghoon KimSung Joong Lee
Oct 1, 2010·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·David J Loane, Kimberly R Byrnes
Feb 1, 2011·Free Radical Biology & Medicine·Daniela SalveminiWilliam L Neumann
Feb 10, 2011·Neurobiology of Disease·Hai ChenPak H Chan
Jun 16, 2011·Nature Reviews. Neuroscience·Samuel David, Antje Kroner
Oct 7, 2011·Journal of Neuroinflammation·Kimberly R ByrnesAlan I Faden
Aug 29, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hongwei QinEtty N Benveniste
Jun 5, 2013·Journal of Neurotrauma·Colton D ThompsonAmgad Hanna
Jul 26, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Junfang WuSusan G Dorsey
Aug 9, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christopher N HansenD Michele Basso
Sep 17, 2013·The Journal of Pain : Official Journal of the American Pain Society·Nicholas S GregoryKathleen A Sluka
Dec 11, 2013·Translational Stroke Research·Wai-Man ChanDamien D Pearse
Dec 18, 2013·Journal of Neuropathology and Experimental Neurology·David J LoaneAlan I Faden
Jun 4, 2014·The European Journal of Neuroscience·Anke Tappe-Theodor, Rohini Kuner

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Citations

Sep 10, 2020·Molecular Neurobiology·Simin TangJun Zhou
Jul 25, 2020·Journal of Orthopaedic Surgery and Research·Jian FuJianhui Zhao
Nov 9, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Luísa Teixeira-SantosDora Pinho
May 1, 2021·International Journal of Molecular Sciences·Karina MaciakJoanna Saluk-Bijak
Nov 11, 2020·Journal of Agricultural and Food Chemistry·Jing LiJun-Qing Huang
Nov 17, 2021·Neural Regeneration Research·Jiaying ZhengLong-Jun Wu

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