Inhibition of nucleoporin member Nup214 expression by miR-133b perturbs mitotic timing and leads to cell death

Molecular Cancer
Sumana BhattacharjyaSusanta Roychoudhury

Abstract

Nucleoporins mediate nucleocytoplasmic exchange of macromolecules and several have been assigned active mitotic functions. Nucleoporins can participate in various mitotic functions like spindle assembly, kinetochore organisation and chromosome segregation- important for genome integrity. Pathways to genome integrity are frequently deregulated in cancer and many are regulated in part by microRNAs. Indeed, altered levels of numerous microRNAs have frequently been associated with tumorigenesis. Here, we unveil a microRNA-mediated regulation of the nucleoporin Nup214 and its downstream effect on genome integrity. Databases/bioinformatic tools such as miRBase, Oncomine and RNAhybrid predicted Nup214 as a miR-133b target. To validate this, we used luciferase reporter assays, Real-Time PCR and immuno-blotting. Flow cytometry and immuno-blots of mitotic markers were used to analyse cell cycle pattern upon thymidine synchronization and miR-133b treatment. Mitotic indices and chromosomal abnormalities were assessed by immuno-fluorescence for FITC-tagged phospho-H3 as well as video-microscopy for GFP-tagged histone H4. Annexin V/propidium iodide staining, caspase3/PARP cleavage and colony formation assays were done to investigate cell dea...Continue Reading

References

Apr 4, 2001·The Journal of Cell Biology·N den Elzen, J Pines
Jul 11, 2003·Developmental Cell·Alexei Arnaoutov, Mary Dasso
Aug 20, 2003·International Journal of Cancer. Journal International Du Cancer·Khalid M MinhasJoseph A Califano
Sep 10, 2003·The Journal of Cell Biology·Davide SalinaBrian Burke
Nov 1, 2003·Nature Cell Biology·P Todd Stukenberg, Ian G Macara
Apr 8, 2004·Neoplasia : an International Journal for Oncology Research·Daniel R RhodesArul M Chinnaiyan
Sep 24, 2004·RNA·Marc RehmsmeierRobert Giegerich
Oct 1, 2005·Nature Reviews. Cancer·Geert J P L KopsDon W Cleveland
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Jun 24, 2009·Nature Reviews. Molecular Cell Biology·Andrew J Holland, Don W Cleveland
Jul 7, 2009·Seminars in Cell & Developmental Biology·Songli Xu, Maureen A Powers
May 26, 2010·Molecular Cancer·Chieko HashizumeRichard W Wong
Oct 5, 2010·Nature Reviews. Drug Discovery·Ramiro GarzonCarlo M Croce
Jan 29, 2011·Cell Cycle·Hiroshi NakanoRichard W Wong
Feb 8, 2011·Cancer Metastasis Reviews·Tatsuyoshi Funasaka, Richard W Wong
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Masayuki KanoHisahiro Matsubara
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Apr 2, 2011·The Journal of Biological Chemistry·Somsubhra NathSusanta Roychoudhury
Jun 21, 2011·Cellular Signalling·Guillaume Chatel, Birthe Fahrenkrog
Jul 10, 2012·Current Opinion in Cell Biology·Naoko Imamoto, Tomoko Funakoshi

❮ Previous
Next ❯

Citations

Apr 20, 2016·International Journal of Molecular Sciences·Azadeh AzizianJochen Gaedcke
May 28, 2016·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Mohammad Iqbal LoneWaseem Ahmad
Nov 1, 2016·Nature Structural & Molecular Biology·Xudong ChenDing Xue
Feb 14, 2019·Human Genetics·Hanan E ShamseldinFowzan S Alkuraya
Oct 28, 2019·Journal of Cancer Research and Clinical Oncology·Adhiraj Roy, Gopeshwar Narayan
Mar 22, 2019·Scientific Reports·Aatira VijayBhuvnesh Kumar
Nov 11, 2017·Cancer Biomarkers : Section a of Disease Markers·Limei FanFei Wu
Jan 24, 2019·Cells·Adélia Mendes, Birthe Fahrenkrog
Feb 22, 2017·International Journal of Molecular Sciences·Angelica Judith Granados-LópezJesús Adrián López
Jul 3, 2020·International Journal of Molecular Sciences·Paulína PidíkovaIveta Herichova

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
FACS
flow cytometry
chromosomal aberrations
transfections
PCR
protein assay
fluorescence-activated cell sorter

Software Mentioned

ImageJ
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.