Inhibition of odontogenic differentiation of human dental pulp cells by dental resin monomers

Biomaterials Research
Ji Hyun KwonHyeong-Cheol Yang

Abstract

Dental resin monomers that are leached from the resin matrix due to incomplete polymerization can affect the viability and various functions of oral tissues and cells. In this study, the effects of triethylene glycol dimethacrylate (TEGDMA) and 2-hydroxyethyl methacrylate (HEMA) on odontogenic differentiation of human dental pulp cells (HDPCs) were examined. To mimic clinical situations, dental pulp cells were treated with resin monomers for 24 h prior to the analysis of alkaline phosphatase (ALP) activity and mRNA expression of genes related to pulp cell differentiation. To elucidate the underlying signaling pathways, regulation of mitogen-activated protein (MAP) kinases by resin monomers was also investigated. The ALP activity of HDPCs was reduced by TEGDMA and HEMA at noncytotoxic concentrations. The mRNA expression of dentin sialophosphoprotein (DSPP), osteocalcin (OCN), and osteopontin (OPN) was also downregulated by resin monomers. However, DSPP expression was not affected by hydrogen peroxide (H2O2). Among the MAP kinases examined, ERK activation (ERK phosphorylation) was not affected by either resin monomers or H2O2, whereas JNK was phosphorylated by TEGDMA and HEMA. Phospho-p38 was upregulated by HEMA, while TEGDMA and...Continue Reading

References

Sep 1, 1995·Journal of Dental Research·S RatanasathienJ B Dennison
Apr 1, 1997·European Journal of Oral Sciences·A Peutzfeldt
Apr 30, 1998·Advances in Cancer Research·T S LewisN G Ahn
May 19, 1998·European Journal of Oral Sciences·W Geurtsen
Sep 16, 1999·Cellular and Molecular Life Sciences : CMLS·L A Tibbles, J R Woodgett
Sep 18, 1999·Free Radical Biology & Medicine·H Wang, J A Joseph
Nov 11, 1999·Journal of Biomedical Materials Research·W GeurtsenG Leyhausen
May 12, 2000·Critical Care Medicine·T ObataM B Yaffe
Jun 6, 2003·European Journal of Oral Sciences·Vibeke Barman MichelsenEinar Solheim
Sep 30, 2003·Journal of Dental Research·V JankeW Geurtsen
Nov 6, 2003·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Preben Hörsted-BindslevAntanas Sidlauskas
Jun 5, 2007·Biomaterials·Kirsten L Van LanduytBart Van Meerbeek
May 12, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Na Ryoung KimHyeong-Cheol Yang
Oct 7, 2010·Journal of Dental Research·N B CramerC N Bowman
May 24, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Na Ryoung KimHyeong-Cheol Yang
Jan 27, 2012·Dental Materials : Official Publication of the Academy of Dental Materials·Athina BakopoulouWerner Geurtsen

❮ Previous
Next ❯

Citations

Jan 27, 2017·Journal of Applied Toxicology : JAT·Hiroyuki InamitsuTakayuki Tsukuba
Dec 7, 2017·Journal of Applied Oral Science : Revista FOB·Zeynep Öncel TorunFerit Avcu
Jun 16, 2018·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Dongyuan LuoYiyang Chen
Sep 20, 2018·Journal of Applied Toxicology : JAT·Elise K TiganiGili Kaufman
Jan 10, 2019·Journal of Clinical Periodontology·Håvard Jostein HaugenGiuseppe Perale
Nov 23, 2016·Biomaterials Research·Joon Woo ChonDong June Chung
Aug 28, 2020·Clinical Oral Investigations·Bálint Viktor LovászGergely Berta
Nov 28, 2019·Stem Cells International·Su-Min LeeHae-Hyoung Lee

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
PCR
electrophoresis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.