Inhibition of oxidative metabolism increases persistent sodium current in rat CA1 hippocampal neurons

The Journal of Physiology
Anna K M Hammarström, P W Gage

Abstract

1. Whole-cell patch-clamp recordings from freshly dissociated rat CA1 neurons revealed a large transient Na+ current (INa,T) and a smaller, inactivation-resistant persistent Na+ current (INa,P). Both currents could be blocked with TTX. 2. The average current densities of INa,T and INa,P in thirty cells were 111.0 +/- 9.62 and 0.87 +/- 0.13 pA pF-1, respectively. 3. Inhibiting oxidative phosphorylation by adding 5 mM sodium cyanide to the pipette solution significantly increased the amplitude of INa,P but had no significant effect on the amplitude of INa,T. 4. Exposing CA1 neurons to hypoxia for more than 7 min caused an increase in the amplitude of INa,P. There was also a delayed decrease in the amplitude of INa,T. 5. INa,P was more sensitive to the Na+ channel blockers TTX and lidocaine than INa,T. The IC50 for the effect of TTX on INa,P was 9.1 +/- 1.2 nM whereas the IC50 for INa,T was 37.1 +/- 1.2 nM, approximately 4-fold higher. Lidocaine (lignocaine; 1 microM) reduced INa,P to 0.24 +/- 0.15 of control (n = 4) whereas INa,T was essentially unaffected (0.99 +/- 0. 11, n = 4). 6. These results show that INa,P is increased when oxidative metabolism is blocked in CA1 neurons. The persistent influx of Na+ through non-inactivatin...Continue Reading

References

Jan 1, 1991·Annals of the New York Academy of Sciences·P K StysB R Ransom
Nov 1, 1990·Trends in Pharmacological Sciences·D Nicholls, D Attwell
Jun 1, 1990·The Journal of General Physiology·C R FrenchP W Gage
May 23, 1985·Neuroscience Letters·C R French, P W Gage
Sep 1, 1995·Trends in Pharmacological Sciences·C P Taylor, B S Meldrum
Nov 1, 1993·Trends in Neurosciences·C P Taylor
Jan 1, 1996·Annual Review of Physiology·W E Crill
Dec 1, 1996·The Journal of Physiology·Y K JuP W Gage

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Citations

Apr 16, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Vanessa BuchholzGertrud Haeseler
Feb 19, 2013·Pflügers Archiv : European journal of physiology·Tomohiro NumataYasuo Mori
Oct 21, 2006·Journal of Computational Neuroscience·H KagerG G Somjen
May 17, 2005·Journal of the Neurological Sciences·David A Bechtold, Kenneth J Smith
Jan 15, 2002·Respiration Physiology·Walter M St -John, Ilya A Rybak
Oct 24, 2002·Neurobiology of Aging·Lucio AnnunziatoMaurizio Taglialatela
Apr 22, 2008·Nature Reviews. Neuroscience·Heinz Beck, Yoel Yaari
Aug 2, 2002·Neuroreport·Francesca SpadoniAlessandro Stefani
Feb 22, 2001·Annual Review of Physiology·J Lopez-BarneoP Ortega-Sáenz
May 27, 2014·Nature Reviews. Neuroscience·Daniela Pietrobon, Michael A Moskowitz
Jul 12, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Nilufar FoadiJörg Ahrens
Feb 11, 2004·Médecine sciences : M/S·Laurent VenanceOlivier Manzoni
Oct 16, 1999·The Journal of Physiology·A K Hammarström, P W Gage
Nov 18, 2000·The Journal of Physiology·A K Hammarström, P W Gage
Sep 2, 2006·The Journal of Physiology·Manuel A Castro-AlamancosYoshie Tawara-Hirata
Feb 24, 2016·Journal of Computational Neuroscience·Bartholomew J BacakYaroslav I Molkov
Dec 9, 2003·The Journal of Physiology·Friederike Bergmann, Bernhard U Keller
May 9, 2008·Journal of Molecular and Cellular Cardiology·Sharon L HaleRobert A Kloner
May 25, 2007·Neuroscience Research·Maria Alexandra RibeiroPedro Freire Costa
Dec 12, 2007·British Journal of Pharmacology·D A Saint
Aug 29, 2012·Annals of Neurology·Elena IsaevaGregory L Holmes
Jan 31, 2017·Pain·Michelle A FarrarCindy S-Y Lin
Apr 28, 2012·Pharmacology·Nilufar FoadiSabine Kästner
Jul 20, 2000·Clinical and Experimental Pharmacology & Physiology·M L Fung

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